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Systèmes modèles pour la réplication d'une information génétique.

Abstract : The goal of this research project is to develop an artificial replicating model system to 'repair' abasic sites within DNA double helices. We will not restore a natural base pair, but introduce a chemical modification that restores duplex stability according to the base facing the abasic site. The opposite base will thus serve to select one "repair reagent" among several possibilities. We amount here to a "information transfer system", or a "replicating system", which is an interesting topic by itself. We would like our system to make use of reactions that are reversible on the time scale of the experiment to allow error correction in the system. We would also like the repair units to react with the DNA backbone without having to cleave it, to make the system more reliable. For our system we chose the reaction of aldehydes with amine type compounds to form imine type products. We chose this group of reactions because they can be made reversible in water at pH 7. The work is divided in three main chapters; in chapter two an overview of relevant literature is given. Chapter three involves the work we did on the repair of natural abasic sites. Chapter four involves the work we did on the repair of artificial abasic sites.
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Contributor : Ecole Polytechnique <>
Submitted on : Friday, July 29, 2005 - 8:00:00 AM
Last modification on : Tuesday, July 10, 2012 - 5:51:37 PM
Long-term archiving on: : Saturday, November 26, 2016 - 9:23:13 PM

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Jeroen Verhage. Systèmes modèles pour la réplication d'une information génétique.. Chimie. Ecole Polytechnique X, 2005. Français. ⟨pastel-00001350⟩

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