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Etude du risque allergique à différentes protéines alimentaires Mise au point de modèle de souris allergiques à l'arachide, à l'albumine, à la caséine et à la colle de poisson

Awatif Lifrani
Abstract : Allergic diseases are a common cause of illness in most industrialized countries. The prevalence of allergic disorders has been increasing in the past 10-15 years. Peanut, cow's milk, eggs and fish are strong allergens frequently found in many food products (flours, wines...). Therefore Codex Alimentarius called for the mandatory and comprehensive labelling of all products prepared with these proteins. Our purpose was to establish animal models producing specific immunoglobulin IgE and IgG1 to some food proteins (peanut, fish, albumin and casein) and developing anaphylactic reactions. First of all, we studied wine allergy in four strains of mice (C3H, CBA, DAB/2 and SJL). After the various steps of clarifying, we showed that the settling, filtration and ageing are important stages for the elimination of the proteins used for clarifying the wines. Then, we studied the cross reactivity of peanut-specific antibodies with lupine in four mouse strains. More specifically, C3H, CBA, SJL and BALB/c mice were sensitized with peanut. We showed that mice of different genetic backgrounds can be sensitized to peanut by ip injection to develop anti-peanut Abs that cross react with lupine. Cross-allergenicity may not directly correlate with the presence of cross-reactive Abs since no clinical symptoms of crossallergenicity were seen after ip challenge with lupine. Lastly, we studied the antibody responses to peanut induced in mice after several routes of sensitization in the presence of several adjuvants. We showed that peanut given in the diet with or without adjuvant did not sensitize C3H and CBA mice or cause any allergies. In conclusion, the ip immunization in the presence of alum as an adjuvant remained the fastest means and most effective way to obtain a model of allergic mice.
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Submitted on : Thursday, September 28, 2006 - 8:00:00 AM
Last modification on : Monday, October 19, 2020 - 10:54:40 AM
Long-term archiving on: : Saturday, November 26, 2016 - 3:54:03 PM

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Awatif Lifrani. Etude du risque allergique à différentes protéines alimentaires Mise au point de modèle de souris allergiques à l'arachide, à l'albumine, à la caséine et à la colle de poisson. Life Sciences [q-bio]. INAPG (AgroParisTech), 2006. English. ⟨NNT : 2006INAP0001⟩. ⟨pastel-00001928⟩

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