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*. Quand-nécessaire, 1 g de L-méthionine (Sigma-Aldrich M9625) est dissous dans 250 ml d'eau MilliQ®(système MilliQ, Millipore) Cette solution est filtrée à l'aide d'une membrane en acétate de cellulose 0, p.2

. Lactose, VWR Prolabo 24 931.293) L-méthionine (Sigma-Aldrich M9625) Eau MilliQ® 20 g 1 ou 6 g 250 ml Après dissolution du lactose et de la L-méthionine, cette solution est filtrée à l'aide d'une membrane en acétate de cellulose 0, Sartolab P20) et additionnée au milieu en surfusion. * 03, p.2

H. Aminex, La ligne est équipée d'une pompe (Waters 510) et d'un injecteur automatique réfrigéré (Waters 717) Les composés sont identifiés à l'aide d'un réfractomètre différentiel, Les sucres et acides organiques ont été analysés sur une colonne échangeuse de cations

D. Le-volume, échantillon injecté est de 20 µ l et les analyses sont réalisées à 65 °C durant 28 min. La phase mobile est constituée d'acide sulfurique H 2 SO 4 à 0,01 N et dispensée à un débit de 0,6 ml/min