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Environnement des bactéries et sensibilité aux biocides : mise au point d'une technique rapide pour déterminer in situ l'efficacité bactéricide d'agents antimicrobiens

Abstract : Controlling the hygiene status of surfaces remains a constant concern in different areas. Indeed, the biofouling of surfaces can be a source of dramatic public health problems in food industries (food toxi-infections) or in hospitals (nosocomial infections). The efficiency of biocides remains variable from one application to the others (especially on adherent micro-organisms), it is necessary to improve the disinfectant formula and/or the associated procedures of disinfection. This improvement involves new test methods that allow rapid assessment of the antibacterial activity of marketed or not products, in conditions closed to the reality. So, through this work, we defined a protocol to determine lethal activity of biocides on sessile bacteria, protocol made up of four principal steps: i) selection of micro-organisms and standardisation of their storage and growth conditions, ii) setting a reproductible adhesion protocol of bacteria on soiled or not surfaces, iii) disinfection test on sessile micro-organisms and iv) optimisation of this protocol for in situ observations and reducing the time needed to obtain results by employing fluorescent markers which give indications on cellular viability.
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https://pastel.archives-ouvertes.fr/pastel-00003703
Contributor : Ecole Agroparistech <>
Submitted on : Monday, June 2, 2008 - 8:00:00 AM
Last modification on : Saturday, June 6, 2020 - 8:29:51 PM
Long-term archiving on: : Friday, September 10, 2010 - 12:27:32 PM

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  • HAL Id : pastel-00003703, version 1
  • PRODINRA : 246691

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Citation

Audrey Allion. Environnement des bactéries et sensibilité aux biocides : mise au point d'une technique rapide pour déterminer in situ l'efficacité bactéricide d'agents antimicrobiens. Sciences du Vivant [q-bio]. ENSIA (AgroParisTech), 2004. Français. ⟨NNT : 2004EIAA0140⟩. ⟨pastel-00003703⟩

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