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Contribution au développement d'un microsystème pour la séparation bidimensionnelle de protéines par électrophorèse

Abstract : In the context of new food allergen detection, a microsystem dedicated to bidimensional electrophoretic protein separation has been developed, using isoelectric focusing (IEF) followed by zone electrophoresis (ZE). Both these separation modes have been investigated in capillary format before down-sizing the results obtained into microsystems. IEF protein separation has first been performed. The use of electrolytes consisting of narrow pH cuts of carrier ampholytes (NC) has proven to increase resolution. Microchip milk protein separation has then been performed. Glass and PDMS microchips and several surface treatments have thus been compared, in particular through electroosmotic flow measurements using an original method.Then, microchip ZE protein separation has been investigated. NC have again been used, due to their low conductivity. We found that they allowed decreasing protein adsorption Milk proteins have been separated within a few seconds after having optimized their derivatization with a fluorescent marker. Several microsystem schemes have then been considered to couple the two separation modes.
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https://pastel.archives-ouvertes.fr/pastel-00004227
Contributor : Ecole Espci Paristech <>
Submitted on : Friday, October 10, 2008 - 8:00:00 AM
Last modification on : Saturday, September 26, 2020 - 11:44:03 PM
Long-term archiving on: : Friday, September 10, 2010 - 1:02:23 PM

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  • HAL Id : pastel-00004227, version 1

Citation

Martine Poitevin. Contribution au développement d'un microsystème pour la séparation bidimensionnelle de protéines par électrophorèse. Chimie. Université Pierre et Marie Curie - Paris VI, 2008. Français. ⟨pastel-00004227⟩

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