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Development of a cell-based assay of the elicitation of the allergic reaction. Assessment of the allergenicity of milk and peanut proteins and of the effects of heat treatments on the allergenitcity of Ara h 1.

Fany Blanc
Abstract : IgE-mediated food allergies to peanut and milk represent a major health issue particularly in children. Cross linking of specific IgE bound on basophils and mast cells by the allergen is a critical step for the elicitation of an allergic reaction. A cell-based assay has been developed to analyse the capacity of an allergen to induce the mediator release by those cells when interacting with IgE bound on their membrane. The assay uses cell line RBL SX-38 developed by JP Kinet derived from rat mast cells engineered to express the human high affinity receptor for IgE. The development of the assay in a 96-well format has required the optimization of various parameters involved in both the cell sensitization step using IgE from peanut allergic patients and the cell activation step by the corresponding allergen. This test has confirmed the degranulation potential of all milk and peanut proteins, and in particular of milk casein and peanut 2S albumins, Ara h 2 and Ara h 6, corroborating the available clinical observations. In the frame of the European program EuroPrevall, we have evaluated the impact of thermal treatments on allergenicity of Ara h 1. Our results have shown that allergenicity of Ara h 1 is decreased when the purified protein is heated in solution, but is increased during roasting of peanut because of interactions between Ara h 1 and food matrix constituents. This cell-based test thus demonstrated to be a relevant tool to study the biological consequences of the interactions between allergens and IgE. It makes possible to better understand the relationship between the structure of a food protein and its allergenicty and, hopefully, to assess the allergenicity of new foods without requiring provocation tests on patients.
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https://pastel.archives-ouvertes.fr/pastel-00004944
Contributor : Ecole Agroparistech <>
Submitted on : Thursday, March 26, 2009 - 8:00:00 AM
Last modification on : Friday, October 23, 2020 - 4:38:08 PM
Long-term archiving on: : Thursday, September 30, 2010 - 8:49:36 PM

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Fany Blanc. Development of a cell-based assay of the elicitation of the allergic reaction. Assessment of the allergenicity of milk and peanut proteins and of the effects of heat treatments on the allergenitcity of Ara h 1.. Life Sciences [q-bio]. AgroParisTech, 2008. English. ⟨NNT : 2008AGPT0078⟩. ⟨pastel-00004944⟩

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