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Etude des mécanismes de pyrolyse en présence d'hydroxyde de tetramethylammonium de composés protéiques modèles. Implication pour la détection de matériel azoté dans la matière organique naturelle et l'identification de macromolécules sources

Nicolas Gallois
Abstract : Despite its implication in many environmental processes, Natural Organic Matter (NOM) is only partially characterised, mainly due to its refractory nature. Amide functions have been revealed by spectroscopic studies, but the molecular structure of the concerned units remains unknown. Pyrolysis coupled to Gas Chromatography and Mass Spectrometry (Py-GC-MS), in the presence of Tetra-Methyl-Ammonium Hydroxyde (TMAH) is considered to be a convenient tool to investigate these structures. For a better understanding of the behaviour of nitrogen-containing structures of NOM upon Py-GC-MS/ TMAH, we used this technique to analyse the 20 proteic amino acids, 17 dipeptides, 4 polypeptides and a protein, the Bovin Serum Albumin (BSA). This study allowed highlighting the main pyrolysis mechanisms undergone by the amino acids (direct methylation, cyclisation, dimerisation, homolysis, decarboxylation and deamination) and the influence of the peptidic bond on these mechanisms. Py-GC-MS/TMAH of dipeptides and polypeptides pointed to the importance of cyclisation products, mainly diketopiperazines and more complex compounds based on 3 amino acids, and homolysis products for aromatic amino acids. Morever, this study allowed creating a database for the pyrolysis products arising from amino acids and dipeptides. Finally, pyrolysis of mixtures of BSA with the main two macromolecules of the biomass (lignin and cellulose) showed that response factors are unfavourable to the protein.
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Submitted on : Friday, April 10, 2009 - 8:00:00 AM
Last modification on : Friday, April 10, 2009 - 8:00:00 AM
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Nicolas Gallois. Etude des mécanismes de pyrolyse en présence d'hydroxyde de tetramethylammonium de composés protéiques modèles. Implication pour la détection de matériel azoté dans la matière organique naturelle et l'identification de macromolécules sources. Chemical Sciences. Chimie ParisTech, 2008. English. ⟨pastel-00005006⟩

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