Development of Molecular Tools for the Identification of Complex Bacterial Flora - Application to the Study of Galliform Gut Flora

Bastien Massias
Abstract : This last decade knew a strong passion for the complex microfloras particularly for the floras associated symbiotically with their host like the digestive tract flora. The digestive floras indeed open many opportunities as well on the medical level as on the industrial and the biotechnological levels. In this manuscript, various works on the digestive flora of Galliforms are presented: study of the antibiotic action mode as growth promoter in chicken, search for substitutes to antibiotics in chicken and search of a putative pathogen of non specific enteritis in turkey. New tools useful for the bacterial identification were also developed in this writing. A new technique of clone screening, named CSbyDG, based on a discrimination of tri-band DGGE profiles, proved its effectiveness to screen clone libraries of 16S rDNA. To allow the regrouping of profile obtained in CSbyDG, a data-processing program coded in Visual Basic Application for Excel was developed. This program, named ProReg XL Tool, is able to regroup identical electrophoretic profiles. Its applicability is thus much broader than the only regrouping of profile of CSbyDG. In the long term, CSbyDG should allow, by various bearings, to identify a bacterium in a few hours.
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Life Sciences [q-bio]. INAPG (AgroParisTech), 2008. English. 〈NNT : 2008AGPT0101〉
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Submitted on : Wednesday, May 27, 2009 - 8:00:00 AM
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Bastien Massias. Development of Molecular Tools for the Identification of Complex Bacterial Flora - Application to the Study of Galliform Gut Flora. Life Sciences [q-bio]. INAPG (AgroParisTech), 2008. English. 〈NNT : 2008AGPT0101〉. 〈pastel-00005056〉

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