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Ecologie microbienne des biofilms présents à la surface des planches d'affinage en bois de l'AOC "Reblochon de Savoie" et effet inhibiteur vis à vis de Listeria monocytogenes

Abstract : Wood has a long tradition as a natural material used in cheese ripening. Dairy products have been implicated in several outbreaks of listeriosis. On account of its ubiquitous nature, Listeria monocytogenes can contaminate cheeses all along the production chain. Protective cultures can help to protect the food environment from this pathogenic bacteria. The aim of the study was to confirm that the wooden ripening shelves could limit the implantation of Listeria monocytogenes. After the study of the environment of wooden ripening shelves in the ripening rooms of the ripening partner, the microbial ecology of the surface of the ripening shelves revealed the predominance of the technological microflora and the low levels of undesirable microflora. At the end of the ripening, the high compatibility of the shelves' surface conditions with microbiological life has been demonstrated. On the contrary, after contact with cheeses, the brushing and drying of the shelves modified the microbial environment, where only technological micro-organisms could grow. An inhibitory effect on the two strains of L. monocytogenes tested was observed on wooden ripening shelves settled by a living microbial biofilm. This inhibitory effect was demonstrated for two steps of the ripening cycle of the shelves, including the step the most exposed to the introduction of L. monocytogenes in the ripening rooms. Several mechanisms have been suggested: competition for nutrient as the main mechanism and the presence of inhibitory substances.
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https://pastel.archives-ouvertes.fr/pastel-00005194
Contributor : Ecole Agroparistech <>
Submitted on : Wednesday, June 17, 2009 - 8:00:00 AM
Last modification on : Saturday, June 6, 2020 - 8:24:31 PM
Long-term archiving on: : Friday, September 10, 2010 - 1:39:16 PM

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  • HAL Id : pastel-00005194, version 1
  • PRODINRA : 246663

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Citation

Claire Mariani. Ecologie microbienne des biofilms présents à la surface des planches d'affinage en bois de l'AOC "Reblochon de Savoie" et effet inhibiteur vis à vis de Listeria monocytogenes. Sciences du Vivant [q-bio]. ENSIA (AgroParisTech), 2007. Français. ⟨NNT : 2007AGPT0089⟩. ⟨pastel-00005194⟩

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