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Metabolic and pathophysiological characterization of a postprandial model of endothelial dysfunction in rats. Potential benefits of a nutritional amino acids supplementation in prevention of cardiovascular risk

Joëlle Magné
Abstract : The alteration in vascular endothelial function is the key event in the pathophysiology of atherosclerosis. In healthy humans, a single high-saturated fat/high sucrose meal induces a series of transient vascular, inflammatory and oxidative disturbances that constitute a complex proatherogenic phenotype. However, little is known on early pathways that occur during the postprandial state. Furthermore, some amino acids have been suspected of having an impact on vascular homeostasis, but there is a paucity of information regarding the effect of dietary protein on the initiation of endothelial dysfunction. We aimed to characterize an animal model of postprandial endothelial dysfunction induced by a high-saturated fat meal (HFM), in healthy rats, in which to delineate the early inflammatory response in adipose tissue, and examine whether the nitric oxide (NO) pathway is impaired in the postprandial setting. Using this model, we have explored whether rapeseed protein, an emergent protein rich in arginine and cysteine may modulate postprandial endothelial dysfunction. We have clearly characterized a rat model of postprandial endothelial dysfunction, featuring oxidative stress, impairment of the NO pathway and low-grade inflammation. We have revealed that inflammatory pathways are activated very early in the visceral adipose tissue, and the HFM markedly impair basal systemic whole-body NO production. Interestingly, modulating the protein source of the HFM has a marked protective effect on postprandial endothelial function in healthy rats. Substituting rapeseed protein for milk protein in an HFM totally suppresses postprandial impairment of vascular reactivity and partially decreases postprandial oxidative stress. These results urge on further studies to examine the clinical relevance of specific protein source, in dietary strategies for the prevention of cardiovascular diseases.
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https://pastel.archives-ouvertes.fr/pastel-00005590
Contributor : Ecole Agroparistech <>
Submitted on : Tuesday, February 23, 2010 - 8:00:00 AM
Last modification on : Monday, October 19, 2020 - 10:56:11 AM
Long-term archiving on: : Thursday, September 16, 2010 - 11:21:15 AM

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Joëlle Magné. Metabolic and pathophysiological characterization of a postprandial model of endothelial dysfunction in rats. Potential benefits of a nutritional amino acids supplementation in prevention of cardiovascular risk. Life Sciences [q-bio]. AgroParisTech, 2009. English. ⟨NNT : 2009AGPT0038⟩. ⟨pastel-00005590⟩

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