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Theses

Génération de gouttes en microfluidique pour l'étude de la cellule unique, l'extraction liquide-liquide et la vectorisation.

Abstract : High-throughput generation of nanoliter-sized droplets in microfluidic channels enables simultaneous monitoring of hundreds of reactions. Encapsulation of a single cell in these droplets opens the way to a better understanding of the heterogeneity present in biological organisms. This thesis first describes the development of a novel microfluidic technology dedicated to a high-throughput gene expression measurement of individual cells isolated in droplets and aims at refining the method of reverse transcription followed by a real-time polymerase chain reaction. In the second part, the droplet technology is used to study mass transfers between the dispersed and the continuous phase, illustrated by two applications of liquid-liquid extraction : one concerning the extraction of zinc, the other dealing with the purification of RNA in a cell. The last part is an introduction to a possible vectorization of active substances by means of phagocytosis using complex micrometer-sized emulsions generated in microsystems.
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https://pastel.archives-ouvertes.fr/pastel-00005739
Contributor : Ecole Espci Paristech <>
Submitted on : Wednesday, February 10, 2010 - 8:00:00 AM
Last modification on : Saturday, September 26, 2020 - 11:44:07 PM
Long-term archiving on: : Friday, October 19, 2012 - 1:00:33 PM

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  • HAL Id : pastel-00005739, version 1

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Pascaline Mary. Génération de gouttes en microfluidique pour l'étude de la cellule unique, l'extraction liquide-liquide et la vectorisation.. Sciences de l'ingénieur [physics]. Université Pierre et Marie Curie - Paris VI, 2009. Français. ⟨pastel-00005739⟩

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