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Détection de peroxyde d'hydrogène résolue en temps à l'aide de nanoparticules Y(1-x)Eu(x)VO(4) luminescentes : application à la signalisation vasculaire

Abstract : Reactive Oxygen Species, especially hydrogen peroxide, play an impor- tant role as messenger molecules for many signaling cascades in cells. Because high concentrations of hydrogen peroxide can have deleterious effects on cells, its regulation is vital. Hence, its time-resolved detection is a first step towards understanding cell mechanisms and efficient medical treatment against diseases like hypertension or atherosclerosis. This PhD work focuses on the development of a new oxidant sensor based on europium-doped yttrium vanadate nanoparticles. These nanoparticles were successfully internalized in vascular smooth muscle cells and can mea- sure the intracellular hydrogen peroxide concentration with a time and space resolution for a broad range of concentrations (typically between 1μM and 5 mM). After sensor calibration, the nanoparticles were used to detect hydro- gen peroxide production in signaling processes elicited by endothelin-1 and platelet-derived growth factor. The two distinct dynamics observed can partially explain why the induced cell response is different.
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https://pastel.archives-ouvertes.fr/pastel-00006271
Contributor : Ecole Polytechnique <>
Submitted on : Thursday, August 19, 2010 - 8:00:00 AM
Last modification on : Wednesday, January 23, 2019 - 10:29:00 AM

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  • HAL Id : pastel-00006271, version 1

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Thanh-Liêm Nguyên. Détection de peroxyde d'hydrogène résolue en temps à l'aide de nanoparticules Y(1-x)Eu(x)VO(4) luminescentes : application à la signalisation vasculaire. Physique [physics]. Ecole Polytechnique X, 2009. Français. ⟨pastel-00006271⟩

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