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Nouvelles architectures de biopuces à base de silicium amorphe

Abstract : Biochips are more and more widely used for genetics or drug-screening applications. DNA chips have been considered here. Single strand DNA probes anchored on a surface hybridize with target molecules present in an assay solution. The recognition event is most often detected by fluorescence. With unlabelled targets, plasmon resonance is generally used for detection. This work has been devoted to the development of new architectures to improve biochips performances. The reliability of probe anchoring to the surface represents a critical issue for state-of-the art biochips. The use of thin layers of amorphous hydrogenated silicon and amorphous silicon-carbon alloys offers a convenient and inexpensive route to improve the reliability and robustness of probe anchoring. This allows for benefiting from recent progress in surface chemistry of silicon, with the possibility to realize direct covalent Si-C bonding. By adjusting the characteristics of the layer, enhanced optical response may be obtained for fluorescence and surface-plasmon resonance detection. Both techniques can be combined into the same reusable sensor, allowing for the detection of highly diluted DNA targets (5 10-15 mol/L) and single mismatch discrimination.
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https://pastel.archives-ouvertes.fr/pastel-00529839
Contributor : Larbi Touahir <>
Submitted on : Tuesday, October 26, 2010 - 5:10:02 PM
Last modification on : Wednesday, March 27, 2019 - 4:18:02 PM
Long-term archiving on: : Thursday, January 27, 2011 - 3:01:15 AM

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  • HAL Id : pastel-00529839, version 1

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Larbi Touahir. Nouvelles architectures de biopuces à base de silicium amorphe. Science des matériaux [cond-mat.mtrl-sci]. Ecole Polytechnique X, 2010. Français. ⟨pastel-00529839⟩

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