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Utilisation de gouttelettes d'émulsion pour l'étude de l'adhésion spécifique entre deux surfaces fluides

Abstract : We have built an experimental model system of specific adhesion between two fluid surfaces on which diffuse ligand and receptor proteins. We have experimentally established the link between macroscopic energy of adhesion end the microscopic motor, the creation of specific links. In order to measure the macroscopic energy of adhesion, we have used emulsion droplets. While adhering, the droplets are deformed. Knowing their surface tension, the increase of surface gives access to the adhesion energy. As a microscopic motor, we have used the model couple streptavidin/biotin. The emulsion droplets are functionalized with these adhesive proteins (streptavidin). Put into contact with supported lipid bilayers carrying the receptors (biotin), the droplets adhere specifically. We have led a static study of this adhesion. With different fluorescence technics (epifluorescence, Fluorescence Correlation Spectroscopy, Fluorescence assays), we have quantified the absolute number of proteins involved in the adhesive patches in order to compare it to adhesion energy. We have seen that adhesion energy is proportional to the energy of one link. Besides, measurement in Reflection Iterference Contrast Microscopy allowed us to study the dynamics of patch growth. We have enlighted a quasi-static diffusive growth. Changing the quantities of proteins involved and their nature (length of the links), we have investigated the different static and dynamic situations of specific adhesion in our model. We have discovered effects of compressibility in adhesive films which brings new views on cell adhesion.
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Submitted on : Monday, January 10, 2011 - 3:43:29 PM
Last modification on : Thursday, November 18, 2021 - 4:02:53 AM
Long-term archiving on: : Monday, April 11, 2011 - 4:20:12 PM


  • HAL Id : pastel-00554288, version 1


Jean-Daniel Emerard. Utilisation de gouttelettes d'émulsion pour l'étude de l'adhésion spécifique entre deux surfaces fluides. Chimie analytique. Université Pierre et Marie Curie - Paris VI, 2010. Français. ⟨pastel-00554288⟩



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