S. Millipore and F. ). Quentin-en-yvelines, Cette étape est réalisée grâce au robot, Genesis Proteam, vol.150

. Après-Évaporation-de-l-'acn, chaque fraction est reprise dans 10L de solvant A, transférée dans un tube LC classique et réinjectée successivement pour une séparation RP-HPLC. Pour l'élimination des contaminants sur le circuit SCX, une cartouche de purification (C18 PepMap100, 5m, 100 Å, 1 mm d.i

. Le-kit-d-'essai-enzcheck, Molecular Probes) contenant de la caséine BODIPY FL comme substrat générique a été utilisé en suivant la procédure du fabricant, i.e. en maintenant un rapport extrait L'incubation dure une nuit à 37°C. Cette étude a été faite à différents pH : tampon acétate 0, p.10

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