&. Abadie and . Prouvost-danon, Specific and total IgE responses to antigenic stimuli in Brown-Norway, Lewis and Sprague-Dawley rats, Immunology, vol.39, pp.561-570, 1980.

T. Ahlers, . Lambert, &. Kuckuk, and . Wiermann, 1H NMR analysis of sporopollenin from Typha Angustifolia, Phytochemistry, vol.50, issue.6, pp.1095-1103, 1999.
DOI : 10.1016/S0031-9422(98)00225-8

. Al-humadi, . Siegel, . Lewis, . Barger, . Ma et al., THE EFFECT OF DIESEL EXHAUST PARTICLES (DEP) AND CARBON BLACK (CB) ON THIOL CHANGES IN PULMONARY OVALBUMIN ALLERGIC SENSITIZED BROWN NORWAY RATS, Experimental Lung Research, vol.28, issue.5, pp.333-382, 2002.
DOI : 10.1080/01902140290091976

. Allegra, . Blasi, . Centanni, . Cosentini, . Denti et al., Acute exacerbations of asthma in adults: role of <I>Chlamydia Pneumoniae</I> infection, European Respiratory Journal, vol.7, issue.12, pp.2165-2168, 1994.
DOI : 10.1183/09031936.94.07122165

&. Andersson and . Lidholm, Characteristics and Immunobiology of Grass Pollen Allergens, International Archives of Allergy and Immunology, vol.130, issue.2, pp.87-107, 2003.
DOI : 10.1159/000069013

&. Anto and . Sunyer, Thunderstorms: a risk factor for asthma attacks, Thorax, vol.52, issue.8, pp.669-70, 1997.
DOI : 10.1136/thx.52.8.669

&. Arts and . Kuper, Animal models to test respiratory allergy of low molecular weight chemicals: A guidance, Methods, vol.41, issue.1, pp.61-71, 2007.
DOI : 10.1016/j.ymeth.2006.07.005

. Bacsi, . Choudhury, . Dharajiya, &. Sur, and . Boldogh, Subpollen particles: Carriers of allergenic proteins and oxidases, Journal of Allergy and Clinical Immunology, vol.118, issue.4, pp.844-850, 2006.
DOI : 10.1016/j.jaci.2006.07.006

. Badorrek, . Larbig, . Dick, . Kock, . Hecker et al., The role of pollen starch granules in bronchial inflammation, Allergy, vol.63, p.65, 2008.

F. Ball, &. Peden, and . Kehrl, Allergen bronchoprovocation of patients with mild allergic asthma after ozone exposure???, ??????, ???, ??????, Journal of Allergy and Clinical Immunology, vol.98, issue.3, pp.563-572, 1996.
DOI : 10.1016/S0091-6749(96)70090-8

. Barragan-meijueiro, &. Morfin-maciel, and . Nava-ocampo, A Mexican population-based study on exposure to paracetamol and the risk of wheezing, rhinitis, and eeczema in childhood, J Investig Allergol Clin Immunol, vol.16, pp.247-52, 2006.

&. Behrendt and . Becker, Localization, release and bioavailability of pollen allergens: the influence of environmental factors, Current Opinion in Immunology, vol.13, issue.6, pp.709-715, 2001.
DOI : 10.1016/S0952-7915(01)00283-7

K. Behrendt, E. Von-eschenbach, . Risse, &. Huss-marp, and . Ring, Secretion of Proinflammatory Eicosanoid-Like Substances Precedes Allergen Release from Pollen Grains in the Initiation of Allergic Sensitization, International Archives of Allergy and Immunology, vol.124, issue.1-3, pp.121-126, 2001.
DOI : 10.1159/000053688

T. Behrendt, . Sliwa-tomczok, E. Kasche, . Von-eschenbach, &. Becker et al., Timothy Grass (<i>Phleum pratense</i> L.) Pollen as Allergen Carriers and Initiators of an Allergic Response, International Archives of Allergy and Immunology, vol.118, issue.2-4, pp.414-422, 1999.
DOI : 10.1159/000024151

. Bellomo, . Gigliotti, . Treloar, . Holmes, . Suphioglu et al., Two consecutive thunderstorm associated epidemics of asthma in the city of Melbourne. The possible role of rye grass pollen, Med J Aust, vol.156, pp.834-841, 1992.

. Bennich, . Ishizaka, R. Johansson, &. Stanworth, and . Terry, Immunoglobulin E. A new class of human immunoglobulin, Immunochemistry, vol.5, issue.4, pp.323-327, 1968.
DOI : 10.1016/0019-2791(68)90128-6

T. Berger and . Lara, Cytokines et allergie immédiate : contrôle de la phase tardive. Revue Française d'Allergologie et d'Immunologie Clinique, pp.298-304, 1996.

. Bernsen, &. Jongste, and . Wouden, Birth order and sibship size as independent risk factors for asthma, allergy, and eczema. Pediatric Allergy and Immunology, pp.464-469, 2003.

. Bessot, Allergènes végétaux non polliniquesPlant derived-allergens (excluding pollen) Revue Française d'Allergologie et d'Immunologie Clinique, pp.40-52, 2003.

. Enerback, The differentiation and maturation of inflammatory cells involved in the allergic response: mast cells and basophils, Allergy, vol.84, issue.1, pp.4-10, 1997.
DOI : 10.1073/pnas.86.14.5542

A. Enriquez, . Davis, . Freels, . Park, &. Hershow et al., The relationship between vaccine refusal and self-report of atopic disease in children, Journal of Allergy and Clinical Immunology, vol.115, issue.4, pp.737-744, 2005.
DOI : 10.1016/j.jaci.2004.12.1128

. Epstein, Are mouse models of allergic asthma useful for testing novel therapeutics? Experimental and Toxicologic Pathology, pp.41-44, 2006.

. Ferreira, . Hawranek, . Gruber, &. Wopfner, and . Mari, Allergic cross-reactivity: from gene to the clinic, Allergy, vol.355, issue.14, pp.243-67, 2004.
DOI : 10.1067/mai.2000.106041

&. Ferrence and . Ashley, Protecting children from passive smoking, BMJ, vol.321, issue.7257, pp.310-311, 2000.
DOI : 10.1136/bmj.321.7257.310

G. Fischer, . Fahlbusch, . Muller, &. Kraft, and . Valenta, Characterization of Phl p 4, a major timothy grass, Journal of Allergy and Clinical Immunology, vol.98, issue.1, pp.189-98, 1996.
DOI : 10.1016/S0091-6749(96)70242-7

. Flöistrup, . Swartz, . Bergström, . Alm, V. Scheynius et al., Allergic disease and sensitization in Steiner school children, Journal of Allergy and Clinical Immunology, vol.117, issue.1, pp.59-66, 2006.
DOI : 10.1016/j.jaci.2005.09.039

R. Gencay, . Tamm, . Soler, &. Perruchoud, and . Roth, Antibodies in Patients with Asthma, American Journal of Respiratory and Critical Care Medicine, vol.163, issue.5, pp.1097-1100, 2001.
DOI : 10.1164/ajrccm.163.5.2003162

. Gern, Viral and bacterial infections in the development and progression of asthma, Journal of Allergy and Clinical Immunology, vol.105, issue.2, pp.497-502, 2000.
DOI : 10.1016/S0091-6749(00)90050-2

. Gilliland, . Berhane, . Mcconnell, . Gauderman, . Vora et al., Maternal smoking during pregnancy, environmental tobacco smoke exposure and childhood lung function, Thorax, vol.55, issue.4, pp.271-277, 2000.
DOI : 10.1136/thorax.55.4.271

URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1745733

. Goetzl, Changing paradigms in the immunological science of allergy, Current Allergy and Asthma Reports, vol.112, issue.1, pp.28-31, 2008.
DOI : 10.1007/s11882-007-0024-0

. Granum, &. Gaarder, and . Løvik, IgE adjuvant effect caused by particles ??? immediate and delayed effects, Toxicology, vol.156, issue.2-3, pp.149-159, 2001.
DOI : 10.1016/S0300-483X(00)00375-9

D. Grote, &. Van-ree, and . Valenta, Immunogold electron microscopic localization of timothy grass (Phleum pratense) pollen major allergens Phl p I and Phl p V after anhydrous fixation in acrolein vapor., Journal of Histochemistry & Cytochemistry, vol.42, issue.3, pp.427-458, 1994.
DOI : 10.1177/42.3.8308259

S. Grote, &. Valenta, and . Reichelt, Group 13 Allergens as Environmental and Immunological Markers for Grass Pollen Allergy: Studies by Immunogold Field Emission Scanning and Transmission Electron Microscopy, International Archives of Allergy and Immunology, vol.136, issue.4, pp.303-313, 2005.
DOI : 10.1159/000083975

. Grote, . Vrtala, . Niederberger, . Wiermann, &. Valenta et al., Release of allergen-bearing cytoplasm from hydrated pollen: A mechanism common to a variety of grass (Poaceae) species revealed by electron microscopy???, Journal of Allergy and Clinical Immunology, vol.108, issue.1, pp.109-115, 2001.
DOI : 10.1067/mai.2001.116431

O. Groux, . 'garra, . Bigler, . Rouleau, D. Antonenko et al., A CD4+ T-cell subset inhibits antigen-specific T-cell responses and prevents colitis, Nature, vol.389, pp.737-779, 1997.

&. Guenounou and . David, Les cytokines dans la physiopathologie et l'exploration de la réponse allergique. Revue Française des Laboratoires, pp.51-56, 2000.

. Guilpain, &. Guillevin, and . Mouthon, Les prot??ines cationiques de??l'??osinophile??: marqueurs d'activation du??polynucl??aire ??osinophile, La Revue de M??decine Interne, vol.27, issue.5, pp.406-414, 2006.
DOI : 10.1016/j.revmed.2006.01.008

. Haby, . Marks, &. Peat, and . Leeder, Daycare attendance before the age of two protects against atopy in preschool age children, Pediatric Pulmonology, vol.153, issue.5, pp.377-384, 2000.
DOI : 10.1002/1099-0496(200011)30:5<377::AID-PPUL3>3.0.CO;2-3

C. Haczku, . Sun, . Barnes, &. Kay, and . Moqbel, Airway hyperresponsiveness, elevation of serum-specific IgE and activation of T cells following allergen exposure in sensitized Brown-Norway rats, Immunology, vol.85, pp.598-603, 1995.

. Haczku, . Moqbel, . Jacobson, . Kay, &. Barnes et al., T-cells subsets and activation in bronchial mucosa of sensitized Brown-Norway rats after single allergen exposure, Immunology, vol.85, pp.591-598, 1995.

. Hallstrand, . Fischer, . Wurfel, . Afari, &. Buchwald et al., Genetic pleiotropy between asthma and obesity in a community-based sample of twins, Journal of Allergy and Clinical Immunology, vol.116, issue.6, pp.1235-1241, 2005.
DOI : 10.1016/j.jaci.2005.09.016

B. Hansen, &. Dekruyff, and . Umetsu, Allergen-specific Th1 cells fail to counterbalance Th2 cell???induced airway hyperreactivity but cause severe airway inflammation, Journal of Clinical Investigation, vol.103, issue.2, pp.175-83, 1999.
DOI : 10.1172/JCI5155

. Hansen, . Mcintire, . Yeung, . Berry, . Thorbecke et al., CD4+ T helper cells engineered to produce latent TGF-??1 reverse allergen-induced airway hyperreactivity and inflammation, Journal of Clinical Investigation, vol.105, issue.1, pp.61-70, 2000.
DOI : 10.1172/JCI7589

S. Hatch, . Boykin, . Hu, &. Miller, and . Gardner, Correlation of effects of inhaled versus intratracheally injected males on susceptibility to respiratory infection in mice, Am Rev Respir Dis, vol.124, pp.167-73, 1981.

T. Heinrich, &. Gehring, and . Thefeld, Traffic at residential address, respiratory health, and atopy in adults: the National German Health Survey 1998, Environmental Research, vol.98, issue.2, pp.240-249, 1998.
DOI : 10.1016/j.envres.2004.08.004

W. Hejl, . Kleine-tebbe, . Johansen, &. Broge, and . Ipsen, Phleum pratense alone is sufficient for allergen specific immunotherapy against allergy to Pooideae grass pollens, World Allergy Organization Journal, vol.&NA;, pp.752-759, 2009.
DOI : 10.1097/01.WOX.0000302018.35580.0c

&. Henderson and . Lowrey, Effect of anesthetic agents on lavage fluid parameters used as indicators of pulmonary injury, Lab Anim Sci, vol.33, pp.60-62, 1983.

. Herz, . Botchkarev, &. Paus, and . Renz, Increased airway responsiveness, allergy-type-I skin responses and systemic anaphylaxis in a humanized-severe combined immuno-deficiency mouse model, Clinical <html_ent glyph="@amp;" ascii="&"/> Experimental Allergy, vol.144, issue.5, pp.478-87, 2004.
DOI : 10.1084/jem.176.2.469

&. Heslop-harrison and . Heslop-harrison, The growth of the grass pollen tube: 1. Characteristics of the polysaccharide particles (?P-particles?) associated with apical growth, Protoplasma, vol.66, issue.1-2, pp.71-80, 1982.
DOI : 10.1007/BF01280217

. Holloway, The genetic basis of atopic asthma, Clinical <html_ent glyph="@amp;" ascii="&"/> Experimental Allergy, vol.99, issue.23, pp.1023-1032, 1999.
DOI : 10.1159/000024098

. Holmes, &. Macary, and . Kemeny, Depletion of CD8+ T Cells following Primary Immunization with Ovalbumin Results in a High and Persistent IgE Response, International Archives of Allergy and Immunology, vol.113, issue.1-3, pp.160-162, 1997.
DOI : 10.1159/000237534

L. Hong, . Soo-young, . Kang-mo, . Jae-won, . Kyu-earn et al., High body mass index and dietary pattern are associated with childhood asthma, Pediatric Pulmonology, vol.25, issue.12, pp.1118-1124, 2006.
DOI : 10.1002/ppul.20372

&. Host and . Halken, Hypoallergenic formulas - when, to whom and how long: after more than 15 years we know the right indication!, Allergy, vol.51, issue.s78, pp.45-52, 2004.
DOI : 10.1097/00005176-199704000-00016

. Hrabina, Immunologie moléculaire des allergènes de pollens de graminées. Expressions, pp.8-12, 2007.

&. Humbert and . Garcia, Éotaxine, chimiokine de premier plan de l'allergie. Revue Française d'Allergologie et d'Immunologie Clinique, pp.62-64, 2004.
DOI : 10.1016/j.allerg.2003.10.015

. Ishizaki, . Koizumi, . Ikemori, &. Ishiyama, and . Kushibiki, Studies of prevalence of Japanese cedar pollinosis among the residents in a densely cultivated area, Ann Allergy, vol.58, pp.265-70, 1987.

. Iwamoto, . Nishino, . Magone, . Whitcup, . Yoshida et al., Experimental immune-mediated blepharoconjunctivitis in rats induced by immunization with ragweed pollen, Graefe's Archive for Clinical and Experimental Ophthalmology, vol.238, issue.4, pp.346-51, 2000.
DOI : 10.1007/s004170050363

. Jaeger, Exposure to grass pollen in Europe, Clinical & Experimental Allergy Reviews, vol.149, issue.1, pp.2-6, 2008.
DOI : 10.1111/j.1472-9733.2008.00125.x

. Jamet, . Botturi, &. Diquet, and . Mollimard, Histamine : le rôle du médiateur. Revue Française d'Allergologie et d'Immunologie Clinique, pp.474-479, 2006.

R. Jansen, . Schouten, . Kraan, . Weiss, &. Timens et al., The Relationship of Skin Test Positivity, High Serum Total IgE Levels, and Peripheral Blood Eosinophilia to Symptomatic and Asymptomatic Airway Hyperresponsiveness, American Journal of Respiratory and Critical Care Medicine, vol.159, issue.3, pp.924-931, 1999.
DOI : 10.1164/ajrccm.159.3.9804024

. Jedrychowski, . Galas, &. Whyatt, and . Perera, The Prenatal Use of Antibiotics and the Development of Allergic Disease in One Year Old Infants. A Preliminary Study, International Journal of Occupational Medicine and Environmental Health, vol.19, issue.1, pp.70-76, 2006.
DOI : 10.2478/v10001-006-0010-0

W. Jeffery, . Nelson, &. Collins, and . Kay, Bronchial Biopsies in Asthma: An Ultrastructural, Quantitative Study and Correlation with Hyperreactivity, American Review of Respiratory Disease, vol.140, issue.6, pp.1745-53, 1989.
DOI : 10.1164/ajrccm/140.6.1745

. Johnston, Viruses and asthma, Allergy, vol.314, issue.10, pp.922-954, 1998.
DOI : 10.1016/S0140-6736(97)02207-1

F. Rylander, &. Mcwilliam, and . Currie, (13)-beta- D-glucan may contribute to pollen sensitivity, Clinical and Experimental Immunology, vol.5, issue.3, pp.383-387, 1999.
DOI : 10.1016/0009-8981(94)90110-4

L. Sabaitis, &. Rop, and . Aaron, Validation of intratracheal instillation as an alternative for aerosol inhalation toxicity testing, Journal of Applied Toxicology, vol.30, issue.2, pp.133-173, 1999.
DOI : 10.1002/(SICI)1099-1263(199903/04)19:2<133::AID-JAT549>3.0.CO;2-9

. Sagai, &. Furuyama, and . Ichinose, Biological effects of diesel exhaust particles (DEP). III. Pathogenesis of asthma like symptoms in mice, Free Radical Biology and Medicine, vol.21, issue.2, pp.199-209, 1996.
DOI : 10.1016/0891-5849(96)00032-9

. Sakaguchi, . Sakaguchi, . Asano, &. Itoh, and . Toda, Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25) Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases, J Immunol, vol.155, pp.1151-64, 1995.

F. Sarlo, &. Gaines, and . Ritz, Respiratory Allergenicity of Detergent Enzymes in the Guinea Pig Intratracheal Test: Association with Sensitization of Occupationally Exposed Individuals, Fundamental and Applied Toxicology, vol.39, issue.1, pp.44-52, 1997.
DOI : 10.1006/faat.1997.2356

. Sarlo, . Ritz, . Fletcher, and . Clark, Proteolytic detergent enzymes enhance the allergic antibody responses of guinea pigs to nonproteolytic detergent enzymes in a mixture: Implications for occupational exposure??????????????????, Journal of Allergy and Clinical Immunology, vol.100, issue.4, pp.480-487, 1997.
DOI : 10.1016/S0091-6749(97)70139-8

. Schappi, . Taylor, . Staff, &. Rolland, and . Suphioglu, Immunologic significance of respirable atmospheric starch granules containing major birch allergen Bet v 1, Allergy, vol.77, issue.5, pp.478-83, 1999.
DOI : 10.1034/j.1398-9995.1999.00838.x

V. Schneider and . Velzen, Kinetics and Quantitation of Eosinophil and Neutrophil Recruitment to Allergic Lung Inflammation in a Brown Norway Rat Model, American Journal of Respiratory Cell and Molecular Biology, vol.17, issue.6, pp.702-712, 1997.
DOI : 10.1165/ajrcmb.17.6.2849

. Sears, . Burrows, . Flannery, &. Herbison, and . Holdaway, Atopy in childhood. I. Gender and allergen related risks for development of hay fever and asthma, Clinical <html_ent glyph="@amp;" ascii="&"/> Experimental Allergy, vol.64, issue.11, pp.941-949, 1993.
DOI : 10.1016/0140-6736(92)91260-F

. Shaheen, . Newson, . Henderson, . Headley, . Stratton et al., Prenatal paracetamol exposure and risk of asthma and elevated immunoglobulin E in childhood, Clinical <html_ent glyph="@amp;" ascii="&"/> Experimental Allergy, vol.55, issue.9, pp.18-25, 2005.
DOI : 10.1016/S0140-6736(98)05104-6

. Shaheen, . Newson, . Sherriff, . Henderson, . Heron et al., Paracetamol use in pregnancy and wheezing in early childhood, Thorax, vol.57, issue.11, pp.958-63, 2002.
DOI : 10.1136/thorax.57.11.958

. Sin, . Spier, . Svenson, . Schopflocher, . Senthilselvan et al., The Relationship Between Birth Weight and Childhood Asthma, Archives of Pediatrics & Adolescent Medicine, vol.158, issue.1, pp.60-64, 2004.
DOI : 10.1001/archpedi.158.1.60

. Singh, . Hough, . Theerakulpisut, . Avjioglu, . Davies et al., Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast., Proceedings of the National Academy of Sciences, vol.88, issue.4, pp.1384-1392, 1991.
DOI : 10.1073/pnas.88.4.1384

. Siroux, . Pin, L. Oryszczyn, &. Moual, and . Kauffmann, Relationships of active smoking to asthma and asthma severity in the EGEA study, European Respiratory Journal, vol.15, issue.3, pp.470-477, 2000.
DOI : 10.1034/j.1399-3003.2000.15.08.x

URL : https://hal.archives-ouvertes.fr/inserm-00780296

. Smith, . Larsen, . Cherniack, . Wenzel, . Voelkel et al., Inflammatory cells and eicosanoid mediators in subjects with late asthmatic responses and increases in airway responsiveness, Journal of Allergy and Clinical Immunology, vol.89, issue.6, pp.1076-84, 1992.
DOI : 10.1016/0091-6749(92)90291-9

. Solomon, Airborne pollen: A brief life, Journal of Allergy and Clinical Immunology, vol.109, issue.6, pp.895-900, 2002.
DOI : 10.1067/mai.2002.125556

D. Southam and . Inman, Distribution of intranasal instillations in mice: effects of volume, time, body position, and anesthesia, American Journal of Physiology - Lung Cellular and Molecular Physiology, vol.282, issue.4, pp.833-839, 2002.
DOI : 10.1152/ajplung.00173.2001

. Stazi, . Sampogna, . Montagano, . Grandolfo, &. Couilliot et al., Early life factors related to clinical manifestations of atopic disease but not to skin-prick test positivity in young children. Pediatric Allergy and Immunology, pp.105-112, 2002.
URL : https://hal.archives-ouvertes.fr/hal-00595550

D. Steerenberg, . Van-doorn, . Middendorp, &. Vos, and . Van-loveren, A POLLEN MODEL IN THE RAT FOR TESTING ADJUVANT ACTIVITY OF AIR POLLUTION COMPONENTS, Inhalation Toxicology, vol.30, issue.12, pp.1109-1131, 1999.
DOI : 10.1159/000234990

. Steerenberg, . Withagen, V. Dormans, and . Dalen, Adjuvant Activity of Various Diesel Exhaust and Ambient Particles in Two Allergic Models, Journal of Toxicology and Environmental Health, Part A, vol.13, issue.2, pp.1421-1460, 2003.
DOI : 10.1080/00984109708984062

. Strachan, Hay fever, hygiene, and household size., BMJ, vol.299, issue.6710, pp.1259-60, 1989.
DOI : 10.1136/bmj.299.6710.1259

. Bacsi, . Attila, . Choudhury, K. Barun, . Dharajiya et al., Subpollen particles: Carriers of allergenic proteins and oxidases, Journal of Allergy and Clinical Immunology, vol.118, issue.4, pp.844-850, 2006.
DOI : 10.1016/j.jaci.2006.07.006

P. Badorrek, M. Larbig, M. Dick, W. Kock, H. Hecker et al., The role of pollen starch granules in bronchial inflammation, Allergy, vol.63, p.65, 2008.

A. Barczyk, E. Sozanska, M. Farnik, I. Stepniak-ziolkiewicz, J. Nizio-masior et al., Increased levels of RANTES in induced sputum of chronic asthma but not in seasonal grass pollen-induced rhinitis, Pneumonol Alergol Pol, vol.74, pp.191-197, 2006.

H. Behrendt and W. M. Beckert, Localization, release and bioavailability of pollen allergens: the influence of environmental factors, Current Opinion in Immunology, vol.13, issue.6, pp.709-715, 2001.
DOI : 10.1016/S0952-7915(01)00283-7

D. E. Bice, J. Seagrave, and F. H. Green, Animal models of asthma: potential usefulness for studying health effects of inhaled particles, Inhal Toxicol, vol.12, pp.829-62, 2000.

I. Boldogh, A. Bacsi, B. K. Choudhury, N. Dharajiya, R. Alam et al., ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation, Journal of Clinical Investigation, vol.115, issue.8, pp.2169-79, 2005.
DOI : 10.1172/JCI24422

D. R. Boverhof, R. Billington, B. B. Gollapudi, J. A. Hotchkiss, S. M. Krieger et al., Respiratory sensitization and allergy: Current research approaches and needs, Toxicology and Applied Pharmacology, vol.226, issue.1, pp.1-13, 2008.
DOI : 10.1016/j.taap.2007.10.008

D. Amato, G. Liccardi, G. Frenguelli, and G. , Thunderstorm-asthma and pollen allergy, Allergy, vol.34, issue.1, pp.11-17, 2007.
DOI : 10.1034/j.1398-9995.2001.00317.x

D. Amato, G. Liccardi, G. Russo, M. Amato, and M. , On the interrelationship between outdoor air pollution and respiratory allergy, Aerobiologia, vol.16, issue.1, pp.1-6, 2000.
DOI : 10.1023/A:1007608510653

S. Fischer, M. Grote, B. Fahlbusch, W. D. Muller, D. Kraft et al., Characterization of Phl p 4, a major timothy grass, Journal of Allergy and Clinical Immunology, vol.98, issue.1, pp.189-98, 1996.
DOI : 10.1016/S0091-6749(96)70242-7

M. Grote, C. Dolecek, R. Van-ree, and R. Valenta, Immunogold electron microscopic localization of timothy grass (Phleum pratense) pollen major allergens Phl p I and Phl p V after anhydrous fixation in acrolein vapor., Journal of Histochemistry & Cytochemistry, vol.42, issue.3, pp.427-458, 1994.
DOI : 10.1177/42.3.8308259

P. K. Jeffery, A. J. Wardlaw, F. C. Nelson, J. V. Collins, and A. B. Kay, Bronchial Biopsies in Asthma: An Ultrastructural, Quantitative Study and Correlation with Hyperreactivity, American Review of Respiratory Disease, vol.140, issue.6, pp.1745-53, 1989.
DOI : 10.1164/ajrccm/140.6.1745

C. Kim, . Keun, S. Kim, . Woo, C. Park et al., Bronchoalveolar lavage cytokine profiles in acute asthma and acute bronchiolitis, Journal of Allergy and Clinical Immunology, vol.112, issue.1, pp.64-71, 2003.
DOI : 10.1067/mai.2003.1618

G. Lacroix, Allergies respiratoires, pollens et polluants = Respiratory allergy, pollen and pollutants. Archives des maladies professionnelles et de l, pp.584-591, 2005.
URL : https://hal.archives-ouvertes.fr/ineris-00961897

O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, Protein measurement with the Folin phenol reagent, J Biol Chem, vol.193, pp.265-75, 1951.

V. Mahler, T. L. Diepgen, O. Kubeta, T. Leakakos, W. Truscott et al., Mutual Boosting Effects of Sensitization with Timothy Grass Pollen and Latex Glove Extract on IgE Antibody Responses in a Mouse Model, Journal of Investigative Dermatology, vol.114, issue.5, pp.1039-1082, 2000.
DOI : 10.1046/j.1523-1747.2000.00967.x

A. C. Motta, J. A. Dormans, G. Peltre, G. Lacroix, F. Y. Bois et al., Intratracheal Instillation of Cytoplasmic Granules from <i>Phleum pratense</i> Pollen Induces IgE- and Cell-Mediated Responses in the Brown Norway Rat, International Archives of Allergy and Immunology, vol.135, issue.1, pp.24-33, 2004.
DOI : 10.1159/000080039

A. C. Motta, M. Marliere, G. Peltre, P. A. Sterenberg, and G. Lacroix, Traffic-Related Air Pollutants Induce the Release of Allergen-Containing Cytoplasmic Granules from Grass Pollen, International Archives of Allergy and Immunology, vol.139, issue.4, pp.294-302, 2006.
DOI : 10.1159/000091600

URL : https://hal.archives-ouvertes.fr/ineris-00963000

A. C. Motta, G. Peltre, J. A. Dormans, C. E. Withagen, G. Lacroix et al., Phleum pratense pollen starch granules induce humoral and cell-mediated immune responses in a rat model of allergy, Clinical <html_ent glyph="@amp;" ascii="&"/> Experimental Allergy, vol.160, issue.2, pp.310-314, 2004.
DOI : 10.1046/j.1365-2249.1999.00829.x

URL : https://hal.archives-ouvertes.fr/ineris-00969855

H. Ormstad, E. C. Groeng, O. Duffort, and M. Lovik, The effect of endotoxin on the production of IgE, IgG1 and IgG2a antibodies against the cat allergen Fel d 1 in mice, Toxicology, vol.188, issue.2-3, pp.309-327, 2003.
DOI : 10.1016/S0300-483X(03)00078-7

R. Rylander, B. Fogelmark, A. Mcwilliam, and A. Currie, (13)-beta- D-glucan may contribute to pollen sensitivity, Clinical and Experimental Immunology, vol.5, issue.3, pp.383-387, 1999.
DOI : 10.1016/0009-8981(94)90110-4

G. F. Schappi, P. E. Taylor, I. A. Staff, J. M. Rolland, and C. Suphioglu, Immunologic significance of respirable atmospheric starch granules containing major birch allergen Bet v 1, Allergy, vol.77, issue.5, pp.478-83, 1999.
DOI : 10.1034/j.1398-9995.1999.00838.x

M. B. Singh, T. Hough, P. Theerakulpisut, A. Avjioglu, S. Davies et al., Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast., Proceedings of the National Academy of Sciences, vol.88, issue.4, pp.1384-1392, 1991.
DOI : 10.1073/pnas.88.4.1384

P. A. Steerenberg, J. A. Dormans, C. C. Van-doorn, S. Middendorp, J. G. Vos et al., A POLLEN MODEL IN THE RAT FOR TESTING ADJUVANT ACTIVITY OF AIR POLLUTION COMPONENTS, Inhalation Toxicology, vol.30, issue.12, pp.1109-1131, 1999.
DOI : 10.1159/000234990

C. Suphioglu, Thunderstorm Asthma Due to Grass Pollen, International Archives of Allergy and Immunology, vol.116, issue.4, pp.253-60, 1998.
DOI : 10.1159/000023953

C. Suphioglu, M. B. Singh, P. Taylor, R. Bellomo, P. Holmes et al., Mechanism of grass-pollen-induced asthma, The Lancet, vol.339, issue.8793, pp.569-72, 1992.
DOI : 10.1016/0140-6736(92)90864-Y

I. Szelenyi, Animal models of bronchial asthma, Inflammation Research, vol.49, issue.12, pp.639-54, 2000.
DOI : 10.1007/s000110050642

P. E. Taylor, R. C. Flagan, A. G. Miguel, R. Valenta, and M. M. Glovsky, Birch pollen rupture and the release of aerosols of respirable allergens, Clinical <html_ent glyph="@amp;" ascii="&"/> Experimental Allergy, vol.110, issue.10, pp.1591-1597, 2004.
DOI : 10.1139/cjb-79-7-757

P. E. Taylor, R. C. Flagan, R. Valenta, and M. M. Glovsky, Release of allergens as respirable aerosols: A link between grass pollen and asthma, Journal of Allergy and Clinical Immunology, vol.109, issue.1, pp.51-57, 2002.
DOI : 10.1067/mai.2002.120759

P. E. Taylor, I. A. Staff, M. B. Singh, and R. B. Knox, Localization of the two major allergens in rye-grass pollen using specific monoclonal antibodies and quantitative analysis of immunogold labelling, The Histochemical Journal, vol.84, issue.5, pp.392-401, 1994.
DOI : 10.1007/BF00160051

S. Till, S. Durham, R. Dickason, D. Huston, J. Bungre et al., IL-13 production by allergen-stimulated T cells is increased in allergic disease and associated with IL-5 but not IFN-gamma expression, Immunology, vol.91, issue.1, pp.53-60, 1997.
DOI : 10.1046/j.1365-2567.1997.00218.x

G. Lacroix, M. Guillam, and C. Ségala, Allergies respiratoires, pollens et polluants = Respiratory allergy, pollen and pollutants. Archives des maladies professionnelles et de l, environnement, vol.66, issue.2, pp.584-91, 2005.
URL : https://hal.archives-ouvertes.fr/ineris-00961897

A. Motta, M. Marliere, G. Peltre, P. Sterenberg, and G. Lacroix, Traffic-Related Air Pollutants Induce the Release of Allergen-Containing Cytoplasmic Granules from Grass Pollen, International Archives of Allergy and Immunology, vol.139, issue.4, pp.14-23, 2006.
DOI : 10.1159/000091600

URL : https://hal.archives-ouvertes.fr/ineris-00963000

C. Suphioglu, M. Singh, P. Taylor, R. Bellomo, P. Holmes et al., Mechanism of grasspollen-induced asthma Birch pollen rupture and the release of aerosols of respirable allergens Release of allergens as respirable aerosols: A link between grass pollen and asthma, Lancet Clin Exp Allergy J Allergy Clin Immunol, vol.3397, issue.109, pp.569-72, 1992.

C. Suphioglu, Thunderstorm Asthma Due to Grass Pollen, International Archives of Allergy and Immunology, vol.116, issue.4, pp.253-60, 1998.
DOI : 10.1159/000023953

G. Schappi, P. Taylor, I. Staff, J. Rolland, C. Suphioglu et al., Immunologic significance of respirable atmospheric starch granules containing major birch allergen Bet v 1, Allergy, vol.77, issue.5, pp.478-83, 1999.
DOI : 10.1034/j.1398-9995.1999.00838.x

A. Motta, J. Dormans, G. Peltre, G. Lacroix, F. Bois et al., Intratracheal Instillation of Cytoplasmic Granules from <i>Phleum pratense</i> Pollen Induces IgE- and Cell-Mediated Responses in the Brown Norway Rat, International Archives of Allergy and Immunology, vol.135, issue.1, pp.24-33, 2004.
DOI : 10.1159/000080039

S. Fischer, M. Grote, B. Fahlbusch, W. Muller, D. Kraft et al., Characterization of Phl p 4, a major timothy grass (Phleum pratense) pollen allergen, J Allergy Clin Immunol, vol.9812, issue.11, pp.189-98, 1996.

M. Grote, C. Dolecek, R. Van-ree, and R. Valenta, Immunogold electron microscopic localization of timothy grass (Phleum pratense) pollen major allergens Phl p I and Phl p V after anhydrous fixation in acrolein vapor., Journal of Histochemistry & Cytochemistry, vol.42, issue.3, pp.427-458, 1994.
DOI : 10.1177/42.3.8308259

M. Grote, I. Swoboda, R. Valenta, and R. Reichelt, Group 13 Allergens as Environmental and Immunological Markers for Grass Pollen Allergy: Studies by Immunogold Field Emission Scanning and Transmission Electron Microscopy, International Archives of Allergy and Immunology, vol.136, issue.4, pp.303-313, 2005.
DOI : 10.1159/000083975

S. Vrtala, S. Fischer, M. Grote, L. Vangelista, A. Pastore et al., Molecular, immunological, and structural characterization of Phl p 6, a major allergen and P-particle-associated protein from Timothy grass (Phleum pratense) pollen, J Immunol, vol.163, pp.5489-96, 1999.

A. Chakra, O. Rogerieux, F. Peltre, G. Sénéchal, H. Lacroix et al., Effects of pollen grains and their cytoplasmic granules on allergic humoral and cellular responses in the rat A pollen model in the rat for testing adjuvant activity of air pollution components, Open Allergy Journal Inhal Toxicol, vol.16, issue.11, pp.1109-1131, 1999.

D. Godfrin, H. Senechal, J. Sutra, J. Busnel, F. Desvaux et al., A modified enzymelinked immunosorbent assay adapted for immunodetection of low amounts of water-insoluble proteins

H. Sénéchal, C. Mayer, L. Mao, J. Desvaux, F. Wal et al., Two-dimensional immunoblot analysis of non-water soluble grass pollen allergens. XXII Congress of the EAACI - Abstract Book Subpollen particles: Carriers of allergenic proteins and oxidases, Journal of Allergy and Clinical Immunology, vol.38220, issue.118, pp.844-50, 2003.

P. Wark, J. Simpson, M. Hensley, and P. Gibson, Airway inflammation in thunderstorm asthma, Clinical <html_ent glyph="@amp;" ascii="&"/> Experimental Allergy, vol.162, issue.12
DOI : 10.1183/09031936.96.09102104

P. Jeffery, A. Wardlaw, F. Nelson, J. Collins, and A. Kay, Bronchial Biopsies in Asthma: An Ultrastructural, Quantitative Study and Correlation with Hyperreactivity, American Review of Respiratory Disease, vol.140, issue.6, pp.1745-53, 1989.
DOI : 10.1164/ajrccm/140.6.1745

C. Kim, S. Kim, C. Park, B. Kim, H. Kang et al., Bronchoalveolar lavage cytokine profiles in acute asthma and acute bronchiolitis, Journal of Allergy and Clinical Immunology, vol.112, issue.1, pp.64-71, 2003.
DOI : 10.1067/mai.2003.1618

S. Till, S. Durham, R. Dickason, D. Huston, J. Bungre et al., IL-13 production by allergen-stimulated T cells is increased in allergic disease and associated with IL-5 but not IFN-gamma expression, Immunology, vol.91, issue.1, pp.53-60, 1997.
DOI : 10.1046/j.1365-2567.1997.00218.x

J. Virchow, J. Walker, C. Hafner, D. Kortsik, C. Werner et al., T cells and cytokines in bronchoalveolar lavage fluid after segmental allergen provocation in atopic asthma, Am J Respir Crit Care Med, vol.151, pp.960-968, 1995.

J. Venge, M. Lampinen, L. Hakansson, S. Rak, and P. Venge, Identification of IL-5 and RANTES as the major eosinophil chemoattractants in the asthmatic lung, Journal of Allergy and Clinical Immunology, vol.97, issue.5, 1996.
DOI : 10.1016/S0091-6749(96)70265-8

A. Barczyk, E. Sozanska, M. Farnik, I. Stepniak-ziolkiewicz, J. Nizio-masior et al., Increased levels of RANTES in induced sputum of chronic asthma but not in seasonal grass polleninduced rhinitis, Pneumonol Alergol Pol, vol.74, pp.191-197, 2006.

P. Badorrek, M. Larbig, M. Dick, W. Kock, H. Hecker et al., The role of pollen starch granules in bronchial inflammation, Allergy, vol.63, p.65, 2008.

I. Boldogh, A. Bacsi, B. Choudhury, N. Dharajiya, R. Alam et al., ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation, Journal of Clinical Investigation, vol.115, issue.8
DOI : 10.1172/JCI24422

R. Rylander, B. Fogelmark, A. Mcwilliam, and A. Currie, (13)-beta- D-glucan may contribute to pollen sensitivity, Clinical and Experimental Immunology, vol.5, issue.3, pp.383-387, 1999.
DOI : 10.1016/0009-8981(94)90110-4

G. Lacroix, Allergies respiratoires, pollens et polluants = Respiratory allergy, pollen and pollutants
DOI : 10.1016/s1775-8785(05)79161-x

URL : https://hal.archives-ouvertes.fr/ineris-00961897

M. Singh, T. Hough, P. Theerakulpisut, A. Avjioglu, S. Davies et al., Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast., Proceedings of the National Academy of Sciences, vol.88, issue.4
DOI : 10.1073/pnas.88.4.1384

C. Suphioglu, Thunderstorm asthma due to grass pollen. Int Arch Allergy Immunol, 1998.

G. Schappi, P. Taylor, I. Staff, J. Rolland, and C. Suphioglu, Immunologic significance of respirable atmospheric starch granules containing major birch allergen Bet v 1, Allergy, vol.77, issue.5, pp.478-83, 1999.
DOI : 10.1034/j.1398-9995.1999.00838.x

A. Motta, M. Marliere, G. Peltre, P. Sterenberg, and G. Lacroix, Traffic-Related Air Pollutants Induce the Release of Allergen-Containing Cytoplasmic Granules from Grass Pollen, International Archives of Allergy and Immunology, vol.139, issue.4, pp.294-302, 2006.
DOI : 10.1159/000091600

URL : https://hal.archives-ouvertes.fr/ineris-00963000

C. Suphioglu, M. Singh, P. Taylor, R. Bellomo, P. Holmes et al., Mechanism of grass-pollen-induced asthma, The Lancet, vol.339, issue.8793, pp.569-72, 1992.
DOI : 10.1016/0140-6736(92)90864-Y

A. Chakra, O. Rogerieux, F. Peltre, G. Sénéchal, H. Lacroix et al., Effects of pollen grains and their cytoplasmic granules on allergic humoral and cellular responses in the rat, Open Allergy Journal

A. Chakra, O. Sutra, J. Rogerieux, F. Peltre, G. Sénéchal et al., Cross-allergenicity between pollen grains and their cytoplasmic granules on Brown Norway rats, World Allergy Organization Journal

A. Bacsi, B. Choudhury, N. Dharajiya, S. Sur, I. Boldogh et al., Subpollen particles: Carriers of allergenic proteins and oxidases, Journal of Allergy and Clinical Immunology, vol.118, issue.4, pp.844-50, 2004.
DOI : 10.1016/j.jaci.2006.07.006

A. Motta, J. Dormans, G. Peltre, G. Lacroix, F. Bois et al., Intratracheal Instillation of Cytoplasmic Granules from <i>Phleum pratense</i> Pollen Induces IgE- and Cell-Mediated Responses in the Brown Norway Rat, International Archives of Allergy and Immunology, vol.135, issue.1, pp.24-33, 2004.
DOI : 10.1159/000080039

A. Currie, G. Stewart, and A. Mcwilliam, Alveolar Macrophages Bind and Phagocytose Allergen- Containing Pollen Starch Granules Via C-Type Lectin and Integrin Receptors: Implications for Airway Inflammatory Disease, The Journal of Immunology, vol.164, issue.7, pp.3878-86, 2000.
DOI : 10.4049/jimmunol.164.7.3878

S. Fischer, M. Grote, B. Fahlbusch, W. Muller, D. Kraft et al., Characterization of Phl p 4, a major timothy grass, Journal of Allergy and Clinical Immunology, vol.98, issue.1, pp.189-98, 1996.
DOI : 10.1016/S0091-6749(96)70242-7

M. Grote, C. Dolecek, R. Van-ree, and R. Valenta, Immunogold electron microscopic localization of timothy grass (Phleum pratense) pollen major allergens Phl p I and Phl p V after anhydrous fixation in acrolein vapor., Journal of Histochemistry & Cytochemistry, vol.42, issue.3, pp.427-458, 1994.
DOI : 10.1177/42.3.8308259

M. Grote, I. Swoboda, R. Valenta, and R. Reichelt, Group 13 Allergens as Environmental and Immunological Markers for Grass Pollen Allergy: Studies by Immunogold Field Emission Scanning and Transmission Electron Microscopy, International Archives of Allergy and Immunology, vol.136, issue.4, pp.303-313, 2005.
DOI : 10.1159/000083975

P. Taylor, I. Staff, M. Singh, and R. Knox, Localization of the two major allergens in rye-grass pollen using specific monoclonal antibodies and quantitative analysis of immunogold labelling, The Histochemical Journal, vol.84, issue.5, 1994.
DOI : 10.1007/BF00160051

S. Vrtala, S. Fischer, M. Grote, L. Vangelista, A. Pastore et al., Molecular, immunological, and structural characterization of Phl p 6, a major allergen and P-particle-associated protein from Timothy grass (Phleum pratense) pollen, J Immunol, vol.163, issue.10, pp.5489-96, 1999.

C. Bittner, B. Grassau, K. Frenzel, and X. Baur, Identification of wheat gliadins as an allergen family related to baker's asthma, Journal of Allergy and Clinical Immunology, vol.121, issue.3, pp.744-753, 2008.
DOI : 10.1016/j.jaci.2007.09.051

H. Yeang, S. Arif, F. Yusof, and E. Sunderasan, Allergenic proteins of natural rubber latex, Methods, vol.27, issue.1, 2002.
DOI : 10.1016/S1046-2023(02)00049-X

D. Bice, J. Seagrave, and F. Green, Animal models of asthma: potential usefulness for studying health effects of inhaled particles, Inhal Toxicol, vol.12, issue.9, pp.829-62, 2000.

D. Boverhof, R. Billington, B. Gollapudi, J. Hotchkiss, S. Krieger et al., Respiratory sensitization and allergy: Current research approaches and needs, Toxicology and Applied Pharmacology, vol.226, issue.1, pp.1-13, 2008.
DOI : 10.1016/j.taap.2007.10.008

P. Steerenberg, J. Dormans, C. Van-doorn, S. Middendorp, J. Vos et al., A POLLEN MODEL IN THE RAT FOR TESTING ADJUVANT ACTIVITY OF AIR POLLUTION COMPONENTS, Inhalation Toxicology, vol.30, issue.12, pp.1109-1131, 1999.
DOI : 10.1159/000234990

I. Szelenyi, Animal models of bronchial asthma, Inflammation Research, vol.49, issue.12, pp.639-54, 2000.
DOI : 10.1007/s000110050642

V. Mahler, T. Diepgen, O. Kubeta, T. Leakakos, W. Truscott et al., Mutual Boosting Effects of Sensitization with Timothy Grass Pollen and Latex Glove Extract on IgE Antibody Responses in a Mouse Model, Journal of Investigative Dermatology, vol.114, issue.5, pp.1039-1082, 2000.
DOI : 10.1046/j.1523-1747.2000.00967.x

F. Rogerieux, D. Godfrin, H. Sénéchal, A. Motta, M. Marlière et al., Modifications of Phleum pratense Grass Pollen Allergens following Artificial Exposure to Gaseous Air Pollutants

D. Godfrin, H. Senechal, J. Sutra, J. Busnel, F. Desvaux et al., A modified enzyme-linked immunosorbent assay adapted for immunodetection of low amounts of water-insoluble proteins, Journal of Immunological Methods, vol.326, issue.1-2, pp.83-92, 2007.
DOI : 10.1016/j.jim.2007.07.008

O. Lowry, N. Rosebrough, A. Farr, and R. Randall, Protein measurement with the Folin phenol reagent, J Biol Chem, vol.193, issue.1, pp.265-75, 1951.

H. Sénéchal, C. Mayer, L. Mao, J. Desvaux, F. Wal et al., Two-dimensional immunoblot analysis of non-water soluble grass pollen allergens. XXII Congress of the EAACI -Abstract Book, p.382, 2003.

S. Su, J. Harris, G. Lau, and S. Han, Aqueous-organic solvent extraction of water-insoluble protein from ragweed pollen, pp.104-116, 1987.

S. Su, G. Lau, and J. Harris, Isolation and characterization of a newly identified antigen from ragweed pollen by organic solvent extraction, 1985.

P. Wurtzen, R. Van-neerven, J. Arnved, H. Ipsen, and S. Sparholt, Dissection of the grass allergen???specific immune response in patients with allergies and control subjects: T-cell proliferation in patients does not correlate with specific serum IgE and skin reactivity????????????, Journal of Allergy and Clinical Immunology, vol.101, issue.2, pp.241-250, 1998.
DOI : 10.1016/S0091-6749(98)70389-6

J. Hope, R. Dearman, I. Kimber, and S. Hopkins, The kinetics of cytokine production by draining lymph node cells following primary exposure of mice to chemical allergens Riffo-Vasquez Y, Spina D. Role of cytokines and chemokines in bronchial hyperresponsiveness and airway inflammation, Immunology. Pharmacol Ther, vol.8394, issue.333, pp.250-5185, 1994.

H. Rosenberg, S. Phipps, and P. Foster, Eosinophil trafficking in allergy and asthma, Journal of Allergy and Clinical Immunology, vol.119, issue.6, pp.1303-1313, 2007.
DOI : 10.1016/j.jaci.2007.03.048

K. Takatsu and H. Nakajima, IL-5 and eosinophilia, Current Opinion in Immunology, vol.20, issue.3, pp.288-94, 2008.
DOI : 10.1016/j.coi.2008.04.001

S. Till, S. Durham, R. Dickason, D. Huston, J. Bungre et al., IL-13 production by allergen-stimulated T cells is increased in allergic disease and associated with IL-5 but not IFN-gamma expression, Immunology, vol.91, issue.1, pp.53-60, 1997.
DOI : 10.1046/j.1365-2567.1997.00218.x

A. Barczyk, E. Sozanska, M. Farnik, I. Stepniak-ziolkiewicz, J. Nizio-masior et al., Increased levels of RANTES in induced sputum of chronic asthma but not in seasonal grass pollen-induced rhinitis, Pneumonol Alergol Pol, vol.74, issue.2, pp.191-197, 2006.

P. Jeffery, A. Wardlaw, F. Nelson, J. Collins, and A. Kay, Bronchial Biopsies in Asthma: An Ultrastructural, Quantitative Study and Correlation with Hyperreactivity, American Review of Respiratory Disease, vol.140, issue.6, 1989.
DOI : 10.1164/ajrccm/140.6.1745

J. Venge, M. Lampinen, L. Hakansson, S. Rak, and P. Venge, Identification of IL-5 and RANTES as the major eosinophil chemoattractants in the asthmatic lung, Journal of Allergy and Clinical Immunology, vol.97, issue.5, pp.1110-1115, 1996.
DOI : 10.1016/S0091-6749(96)70265-8

P. Wark, J. Simpson, M. Hensley, and P. Gibson, Airway inflammation in thunderstorm asthma, Clinical <html_ent glyph="@amp;" ascii="&"/> Experimental Allergy, vol.162, issue.12, pp.1750-1756, 2002.
DOI : 10.1183/09031936.96.09102104

S. Wright, P. Hockey, G. Enhorning, P. Strong, K. Reid et al., Altered airway surfactant phospholipid composition and reduced lung function in asthma, J Appl Physiol, vol.89, issue.4, pp.1283-92, 2000.

P. Badorrek, M. Larbig, M. Dick, W. Kock, H. Hecker et al., The role of pollen starch granules in bronchial inflammation ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation, Allergy. J Clin Invest, vol.63115, issue.8, pp.2169-79, 2005.

R. Rylander, B. Fogelmark, A. Mcwilliam, and A. Currie, 1-->3)-beta-D-glucan may contribute to pollen sensitivity383-4. 3. 2 D gel analysis of Phleum pratense PCG water-soluble extract Thirty spots were revealed on the silver stained 2 D gel (figure 3 A) Most of these protein spots, Clin Exp Immunol, vol.115, issue.3, 1999.

. Moreover, neutral spots (pI from 6.0 to 7.3) were located around 2 different Mr ranges, pp.25-26

. Moreover, acidic allergen spots (pI from 4.6 to 6.0) were also detected on a very low Mr (< n°1 and n°8 presented the same patterns. All IgE binding proteins (Mr 35-40, pI 5.3-8.6) revealed by these 2 sera were present on the PVDF revealed by patient serum n°6

R. 1. Davies, R. Rusznak, C. Devalia, and J. , Why is allergy increasing? - environmental factors, Clinical & Experimental Allergy, vol.53, issue.S6, pp.8-14, 1998.
DOI : 10.1046/j.1365-2222.1998.0280s6008.x

M. Singh, T. Hough, P. Theerakulpisut, A. Avjioglu, S. Davies et al., Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast., Proceedings of the National Academy of Sciences, vol.88, issue.4, pp.1384-1392, 1991.
DOI : 10.1073/pnas.88.4.1384

C. Suphioglu, Thunderstorm Asthma Due to Grass Pollen, International Archives of Allergy and Immunology, vol.116, issue.4, pp.253-60, 1998.
DOI : 10.1159/000023953

R. Bellomo, P. Gigliotti, A. Treloar, P. Holmes, C. Suphioglu et al., Two consecutive thunderstorm associated epidemics of asthma in the city of Melbourne. The possible role of rye grass pollen, Med J Aust, vol.156, pp.834-841, 1992.

G. Schappi, P. Taylor, I. Staff, J. Rolland, and C. Suphioglu, Immunologic significance of respirable atmospheric starch granules containing major birch allergen Bet v 1, Allergy, vol.77, issue.5, pp.478-83, 1999.
DOI : 10.1034/j.1398-9995.1999.00838.x

C. Suphioglu, M. Singh, P. Taylor, R. Bellomo, P. Holmes et al., Mechanism of grass-pollen-induced asthma, The Lancet, vol.339, issue.8793, pp.569-72, 1992.
DOI : 10.1016/0140-6736(92)90864-Y

A. Chehregani, A. Majde, M. Moin, M. Gholami, A. Shariatzadeh et al., Increasing allergy potency of Zinnia pollen grains in polluted areas, Ecotoxicology and Environmental Safety, vol.58, issue.2, pp.267-72, 2004.
DOI : 10.1016/j.ecoenv.2003.12.004

H. Sénéchal, C. Mislin-bocquel, F. Desvaux, A. Verhille, and G. Peltre, Pollen grains and urban air pollution: a three year study, Allergy, vol.56, p.46, 2001.

F. Rogerieux, D. Godfrin, H. Senechal, A. Motta, M. Marliere et al., Modifications of <i>Phleum pratense</i> Grass Pollen Allergens following Artificial Exposure to Gaseous Air Pollutants (O<sub>3</sub>, NO<sub>2</sub>, SO<sub>2</sub>), International Archives of Allergy and Immunology, vol.143, issue.2, pp.127-161, 2007.
DOI : 10.1159/000099079

R. Jaenicke, Abundance of Cellular Material and Proteins in the Atmosphere, Science, vol.308, issue.5718, p.73, 2005.
DOI : 10.1126/science.1106335

C. Bittner, B. Grassau, K. Frenzel, and X. Baur, Identification of wheat gliadins as an allergen family related to baker's asthma, Journal of Allergy and Clinical Immunology, vol.121, issue.3, pp.744-753, 2008.
DOI : 10.1016/j.jaci.2007.09.051

D. Godfrin, H. Sénéchal, J. Sutra, J. Busnel, F. Desvaux et al., A modified enzyme-linked immunosorbent assay adapted for immunodetection of low amounts of water-insoluble proteins, Journal of Immunological Methods, vol.326, issue.1-2, pp.83-92, 2007.
DOI : 10.1016/j.jim.2007.07.008

T. Batard, B. Basuyaux, P. Lambin, C. Bremard-oury, R. Hamilton et al., Isotypic Analysis of Grass Pollen-Specific Immunoglobulins in Human Plasma, International Archives of Allergy and Immunology, vol.100, issue.1, pp.68-78, 1993.
DOI : 10.1159/000236390

A. Görg, W. Weiss, and M. Dunn, Current two-dimensional electrophoresis technology for proteomics, PROTEOMICS, vol.20, issue.12, pp.3665-85, 2004.
DOI : 10.1002/pmic.200401031

V. Brodard, B. David, A. Gorg, and G. Peltre, Two-Dimensional Gel Electrophoretic Analysis with Immobilized pH Gradients of <i>Dactylis glomerat</i><i>a</i> Pollen Allergens, International Archives of Allergy and Immunology, vol.102, issue.1, pp.72-80, 1993.
DOI : 10.1159/000236553

P. Righetti and A. Bossi, Isoelectric focusing in immobilized pH gradients: an update, Journal of Chromatography B: Biomedical Sciences and Applications, vol.699, issue.1-2, pp.77-89, 1997.
DOI : 10.1016/S0378-4347(96)00151-X

H. Blum, H. Beier, and H. Gross, Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels, Electrophoresis, vol.156, issue.2, pp.93-102, 1987.
DOI : 10.1002/elps.1150080203

A. Chakra, O. Sutra, J. Desvaux, F. Peltre, G. Lacroix et al., Role of watersoluble and water-insoluble allergens of pollen cytoplasmic granules in humoral and cellular allergic responses. Compte-rendu de l, Académie des Sciences

K. Andersson and J. Lidholm, Characteristics and Immunobiology of Grass Pollen Allergens, International Archives of Allergy and Immunology, vol.130, issue.2, pp.87-107, 2003.
DOI : 10.1159/000069013

C. Guerin-marchand, H. Senechal, A. Bouin, V. Leduc-brodard, G. Taudou et al., Cloning, sequencing and immunological characterization of Dac g 3, a major allergen from Dactylis glomerata pollen, Molecular Immunology, vol.33, issue.9, pp.797-806, 1996.
DOI : 10.1016/0161-5890(96)00015-6

V. Leduc-brodard, F. Inacio, M. Jaquinod, E. Forest, B. David et al., Characterization of Dac g 4, a major basic allergen from Dactylis glomerata pollen????????????, Journal of Allergy and Clinical Immunology, vol.98, issue.6, pp.1065-72, 1996.
DOI : 10.1016/S0091-6749(96)80193-X

A. Chakra, O. Rogerieux, F. Peltre, G. Sénéchal, H. Lacroix et al., Effects of pollen grains and their cytoplasmic granules on allergic humoral and cellular responses in the rat particles: Carriers of allergenic proteins and oxidases, Open Allergy Journal. submitted; Journal of Allergy and Clinical Immunology, vol.23118, pp.844-50, 2006.

A. Motta, J. Dormans, G. Peltre, G. Lacroix, F. Bois et al., Intratracheal Instillation of Cytoplasmic Granules from <i>Phleum pratense</i> Pollen Induces IgE- and Cell-Mediated Responses in the Brown Norway Rat, International Archives of Allergy and Immunology, vol.135, issue.1, pp.24-33, 2004.
DOI : 10.1159/000080039

A. Currie, G. Stewart, and A. Mcwilliam, Alveolar Macrophages Bind and Phagocytose Allergen- Containing Pollen Starch Granules Via C-Type Lectin and Integrin Receptors: Implications for Airway Inflammatory Disease, The Journal of Immunology, vol.164, issue.7, pp.3878-86, 2000.
DOI : 10.4049/jimmunol.164.7.3878

A. Chakra, O. Sutra, J. Rogerieux, F. Peltre, G. Sénéchal et al., Cross-allergenicity between pollen grains and their cytoplasmic granules on Brown Norway rats, World Allergy Organization Journal

V. Leduc-brodard, B. David, and G. Peltre, Comparison of three horizontal two-dimensional electrophoretic techniques to separate grass pollen allergens, Cell Mol Biol, vol.40, pp.1-8, 1994.

H. Sénéchal, C. Mayer, L. Mao, J. Desvaux, F. Wal et al., Two-dimensional immunoblot analysis of non-water soluble grass pollen allergens. XXII Congress of the EAACI -Abstract Book, 2003.

S. Su, J. Harris, G. Lau, and S. Han, Aqueous-organic solvent extraction of water-insoluble protein from ragweed pollen, Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi, vol.20, pp.104-116, 1987.

S. Su, G. Lau, and J. Harris, Isolation and characterization of a newly identified antigen from ragweed pollen by organic solvent extraction, Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi, vol.18, pp.157-68, 1985.

H. Yeang, S. Arif, F. Yusof, and E. Sunderasan, Allergenic proteins of natural rubber latex, Methods, vol.27, issue.1, pp.32-45, 2002.
DOI : 10.1016/S1046-2023(02)00049-X

M. Grote, C. Dolecek, R. Van-ree, and R. Valenta, Immunogold electron microscopic localization of timothy grass (Phleum pratense) pollen major allergens Phl p I and Phl p V after anhydrous fixation in acrolein vapor., Journal of Histochemistry & Cytochemistry, vol.42, issue.3, pp.427-458, 1994.
DOI : 10.1177/42.3.8308259

S. Vrtala, S. Fischer, M. Grote, L. Vangelista, A. Pastore et al., Molecular, immunological, and structural characterization of Phl p 6, a major allergen and P-particleassociated protein from Timothy grass (Phleum pratense) pollen, J Immunol, vol.163, pp.5489-96, 1999.

P. Taylor, I. Staff, M. Singh, and R. Knox, Localization of the two major allergens in rye-grass pollen using specific monoclonal antibodies and quantitative analysis of immunogold labelling, The Histochemical Journal, vol.84, issue.5, pp.392-401, 1994.
DOI : 10.1007/BF00160051

S. Fischer, M. Grote, B. Fahlbusch, W. Muller, D. Kraft et al., Characterization of Phl p 4, a major timothy grass, Journal of Allergy and Clinical Immunology, vol.98, issue.1, pp.189-98, 1996.
DOI : 10.1016/S0091-6749(96)70242-7

M. Grote, I. Swoboda, R. Valenta, and R. Reichelt, Group 13 Allergens as Environmental and Immunological Markers for Grass Pollen Allergy: Studies by Immunogold Field Emission Scanning and Transmission Electron Microscopy, International Archives of Allergy and Immunology, vol.136, issue.4, pp.303-313, 2005.
DOI : 10.1159/000083975

A. Petersen, G. Schramm, M. Schlaak, and W. Becker, Post-translational modifications influence IgE reactivity to the major allergen Phl p 1 of timothy grass pollen, Clinical <html_ent glyph="@amp;" ascii="&"/> Experimental Allergy, vol.203, issue.3, pp.315-336, 1998.
DOI : 10.1016/0167-5699(89)90140-0

E. Batanero, J. Crespo, R. Monsalve, M. Martin-esteban, M. Villalba et al., IgE-binding and histamine-release capabilities of the main carbohydrate component isolated from the major allergen of olive tree pollen, Ole e 1, Journal of Allergy and Clinical Immunology, vol.103, issue.1, pp.147-53, 1999.
DOI : 10.1016/S0091-6749(99)70538-5