Abstract : European agro-environmental policies target several environmental resources used for farming. Difficulties in considering multiple agro-environmental functions (AEF) and farming activities, along with lack of databases, hinder the implementation of such policies at regional scale. Hence we propose a method to qualify AEF fulfilment at landscape and regional scales. Starting from the relationships among farming systems, agro-environmental processes and land use patterns, we suppose a link between land use patterns and AEF fulfilment obtained from bio-physical conditions influencing such processes. The method was multiple functions oriented, because of the current agro-environmental policies; at multiple levels, because of the complex relationships between our research subjects; spatially-explicit, to be reproducible for different time spans; empirical, because partially based on assumed relationships between descriptors of AEF fulfilment. We have tested the method in two European NUTS3: Puy-de-Dome (France) and Grosseto Province (Italy), presenting similar agro-environmental heterogeneity (e.g. varying from arable crops plains to extensive livestock mountains) and different conditions (e.g. soil quality, land use, climate). Even though facing some different AEF, both areas presented a good correspondence between the AEF fulfilment at the farming region and landscape scales. In the French case, we found a higher composition variability of AEF fulfilment but a small spatial variability resulting in a quite homogeneous contribution of farms to AEF and in a poor added value of land use patterns. In the Italian case, we found a correspondence between land use patterns and AEF fulfilment resulting in an high predictive value of land use patterns, overcoming farm technical data. We conclude that land use patterns may be a promising tool to assess AEF only when there is a high AEF spatial variability, which is typical of Mediterranean areas.