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B. Preparation and B. , allo-antisera (antibodies) 1. Bleed a donor chicken for 8 mL of blood and stabilise the, 8% Natrium Citrate. 2. Purify the white blood cells (WBC) on a Lymphoprep gradient according to the manufacturer's description

W. E. Briles, R. M. Goto, C. Auffray, and M. M. Miller, A polymorphic system related to but genetically independent of the chicken major histocompatibility complex, Immunogenetics, vol.37, issue.6, pp.408-422, 1993.
DOI : 10.1007/BF00222464

N. Bumstead, R. Reece, and J. Cook, Genetic Differences in Susceptibility of Chicken Lines to Infection with Infectious Bursal Disease Virus, Poultry Science, vol.72, issue.3, pp.189-193, 1993.
DOI : 10.3382/ps.0720403

P. F. Cotter, R. Taylor, and H. Abplanalp, B-complex associated immunity to Salmonella enteritidis challenge in congenic chickens, Poultry Science, vol.77, issue.12, pp.1846-1851, 1998.
DOI : 10.1093/ps/77.12.1846

V. Fillon, R. Zoorob, M. Yerle, C. Auffray, and A. Vignal, Mapping of the genetically independent chicken major histocompatibility complexes <i>B</i><sup>@</sup> and <i>RFP-</i><i>Y</i><sup>@</sup> to the same microchromosome by two-color fluorescent in situ hybridization, Cytogenetic and Genome Research, vol.75, issue.1, pp.7-9, 1996.
DOI : 10.1159/000134445

F. Guillemot, J. F. Kaufman, K. Skjødt, and C. Auffray, The major histocompatibility complex in the chicken, Trends in Genetics, vol.5, pp.300-304, 1989.
DOI : 10.1016/0168-9525(89)90112-1

H. Juul-madsen, J. Hedemand, J. Salomonsen, and M. Simonsen, Restriction fragment length polymorphism analysis of the chicken B-F and B-L genes and their association with serologically defined B haplotypes, Animal Genetics, vol.20, issue.4, pp.243-247, 1993.
DOI : 10.1111/j.1365-2052.1993.tb00306.x

H. Juul-madsen, M. Afanassieff, and T. Dalgaard, Molecular characterization of major and minor MHC class I and II genes in B21-like haplotypes in chickens, Animal Genetics, vol.23, issue.4, pp.252-261, 2000.
DOI : 10.1016/0161-5890(86)90015-5

H. Juul-madsen, O. Nielsen, T. Krogh-meibom, C. Rontved, T. Dalgaard et al., Major histocompatibility complex-linked immune response of young chickens vaccinated with an attenuated live infectious bursal disease virus vaccine followed by an infection, Poultry Science, vol.81, issue.5, pp.649-656, 2002.
DOI : 10.1093/ps/81.5.649

J. Kaufman, J. Salomonsen, and K. Skjødt, B-G cDNA clones have multiple small repeats and hybridize to both chicken MHC regions, Immunogenetics, vol.14, issue.6, pp.440-51, 1989.
DOI : 10.1007/BF02421176

J. Kaufman and H. Wallny, Chicken MHC Molecules, Disease Resistance and the Evolutionary Origin of Birds, Current Topics in Microbiological Immunology, vol.212, pp.129-141, 1996.
DOI : 10.1007/978-3-642-80057-3_12

G. Kroemer, F. Guillemot, and C. Auffray, Genetic organization of the chicken MHC, Immunologic Research, vol.28, issue.1, pp.8-19, 1990.
DOI : 10.1007/BF02918475

D. Lahiri, N. Jr, and . Ji, A rapid non-enzymatic method for the preparation of HMW DNA from blood for RFLP studies, Nucleic Acids Research, vol.19, issue.19, p.5444, 1991.
DOI : 10.1093/nar/19.19.5444

S. Lamont, The chicken major histocompatibility complex and disease, Revue Scientifique et Technique de l'OIE, vol.17, issue.1, pp.128-142, 1998.
DOI : 10.20506/rst.17.1.1090

S. J. Lamont, C. Bolin, and N. Cheville, Genetic resistance to fowl cholera is linked to the major histocompatibility complex, Immunogenetics, vol.5, issue.suppl. 1, pp.284-289, 1987.
DOI : 10.1007/BF00404420

H. S. Lillehoj, M. Ruff, L. Bacon, S. Lamont, and T. Jeffers, Genetic control of immunity to Eimeria tenella. Interaction of MHC genes and non-MHC linked genes influences levels of disease susceptibility in chickens, Veterinary Immunology and Immunopathology, vol.20, issue.2, pp.135-148, 1989.
DOI : 10.1016/0165-2427(89)90094-9

M. M. Miller, R. Goto, A. Bernot, R. Zoorob, C. Auffray et al., Two Mhc class I and two Mhc class II genes map to the chicken Rfp-Y system outside the B complex., Proceedings of the National Academy of Science USA 91, pp.4397-401, 1994.
DOI : 10.1073/pnas.91.10.4397

J. Salomonsen, D. Dunon, K. Skjødt, T. D. Vainio, O. Kaufman et al., Chicken major histocompatibility complex-encoded B-G antigens are found on many cell types that are important for the immune system., Proceedings of the National Academy of Sciences, vol.88, issue.4, pp.1359-1363, 1991.
DOI : 10.1073/pnas.88.4.1359

J. Sambrook, E. Fritsch, and T. Maniatis, Molecular Cloning ? A Laboratory Manual, 1989.

L. Schierman and C. Lw, Influence of the Major Histocompatibility Complex on Tumor Regression and Immunity in Chickens, Poultry Science, vol.66, issue.5, pp.812-818, 1987.
DOI : 10.3382/ps.0660812

M. Simonsen, Serological identification of the major genetic locus, Acta Pathol Microbiol Scand, vol.83, pp.1-14, 1975.

B. H. Yoo and S. Bl, Association of the major histocompatibility complex with avian leukosis virus infection in chickens, British Poultry Science, vol.66, issue.3, pp.613-620, 1992.
DOI : 10.1080/00071669108417357

R. Zoorob, C. Auffray, and A. Chaussé, Réactifs et méthodes pour la detection des genes lies au CMH d'oiseaux d'élevage tels que le poulet, 1998.