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A global approach of chicken genetic diversity in Taiwan combining phenotypes and molecular markers

Abstract : This thesis deals with genetic diversity of six local chicken breeds in Taiwan. The first objective is to combine phenotypic data (growth and immune response) with molecular data (microsatellite markers, mtDNA, MC1R and LEI0258 marker of MHC) to analyze genetic diversity of six chicken breeds conserved in National Chung Hsing University since 1982. A multivariate method is proposed to combine continuous variables (performance traits) and allelic frequencies (molecular data). The combined analysis clearly discriminates all breeds except Ju-Chi and Quemoy which appear very similar except for immune response. A selection process has been observed on the MC1R gene which controls plumage colour. The second objective is to characterize chicken MHC polymorphism, as described by the hypervariable LEI0258 marker, and study its effect on immune responses. On a total of seventeen LEI0258 alleles, seven were new. The polymorphism at the MHC locus can serve as a useful indicator of genetic diversity for monitoring conservation of small populations. A challenge experiment with a low pathogenic avian influenza virus showed significant differences between breeds, with a faster antibody response in the Quemoy breed and a higher sensitivity to the infection for the Hsin-Yi breed. Breeds also differed in their secondary response to several vaccines, with most often a higher response in the Quemoy breed. Only four out of nineteen MHC haplotypes were shared between breeds and did not influence antibody response but breed specific MHC alleles could influence immune response, particularly in the Quemoy breed. A refined characterization of MHC alleles with a dedicated SNP chip is recommended.
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https://pastel.archives-ouvertes.fr/pastel-00602320
Contributor : Chi-Sheng Chang <>
Submitted on : Wednesday, June 22, 2011 - 3:51:25 AM
Last modification on : Saturday, June 6, 2020 - 5:56:18 AM
Long-term archiving on: : Friday, September 23, 2011 - 2:20:36 AM

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  • HAL Id : pastel-00602320, version 1
  • PRODINRA : 246132

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Citation

Chi-Sheng Chang. A global approach of chicken genetic diversity in Taiwan combining phenotypes and molecular markers. Animal production studies. AgroParisTech, 2011. English. ⟨NNT : 2011AGPT0021⟩. ⟨pastel-00602320⟩

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