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Etude de la diversité génétique d'Aspergillus fumigatus et de Chlamydophila psittaci chez les oiseaux et mise au point de modèles expérimentaux aviaires

Abstract : The filamentous fungus Aspergillus fumigatus and the bacteria Chlamydophila psittaci are two major pathogens in poultry. Although phylogenetically distant, these two microorganisms share the same aerial transmission. Mortality and morbidity associated to aspergillosis and chlamydiosis are responsible for economic losses, in particular in turkey farms. In France, investigations on human cases of chlamydiosis frequently reveal a link with duck farms. The bacteria C. psittaci is carried in the gut of ducks without any clinical signs. In order to investigate the circulation of Aspergillus fumigatus and Chlamydophila psittaci in avian farms, we studied the genetic diversity of the two pathogens. Two new MLVA (Multiple Locus VNTR Analysis) typing methods were set up. These methods revealed to be very discriminant, rapid and easy to use. In the case of A. fumigatus, the MLVA method made it possible to cluster the genotypes according to their geographic origin. It also permitted the analysis of contamination modes in a turkey hatchery. In order to analyze the pathogenicity of Aspergillus fumigatus and Chlamydophila psittaci in birds, two experimental infection models were developed. The first model was designed to reproduce aspergillosis in one-day old chickens inoculated with airborne A. fumigatus conidia. During this experiment, the impact of immunosuppression and of chicken lineage was evaluated. For C. psittaci, a model reproducing an intestinal carriage of the bacteria in one-day old mule ducks was obtained.
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https://pastel.archives-ouvertes.fr/pastel-00603770
Contributor : Jonga Dequeant <>
Submitted on : Monday, June 27, 2011 - 1:12:30 PM
Last modification on : Tuesday, October 20, 2020 - 10:21:37 AM
Long-term archiving on: : Wednesday, September 28, 2011 - 2:22:50 AM

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  • HAL Id : pastel-00603770, version 1
  • PRODINRA : 246319

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Simon Thierry. Etude de la diversité génétique d'Aspergillus fumigatus et de Chlamydophila psittaci chez les oiseaux et mise au point de modèles expérimentaux aviaires. Microbiologie et Parasitologie. AgroParisTech, 2011. Français. ⟨NNT : 2011AGPT0015⟩. ⟨pastel-00603770⟩

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