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Développement d'un microsystème bioanalytique intégrant des nanoparticules magnétiques dédié au diagnostic de l'allergie

Abstract : In the field of allergy diagnosis, the most used in vitro approach consists in quantifying Immunoglobuline E (IgE) in patient sera using enzyme linked immunosorbent assay (ELISA) in conventional microtiterplate. The immunochemical techniques have been chosen since they provide high specificity and sensitivity. Although these approaches are easy to use and allow automation, they required high sample volume (100 μL) and suffer from mass transfer limitations which lead to long assay time. In that way, we have developed a bioanalytical microsystem integrating magnetic nanoparticles dedicated to milk allergy diagnosis. The nanoparticles, previously functionalized with the capture molecule (antigen in our case) were used as immunosupport thus allowing target (IgE) capture in colloidal phase. The reduction of the immunosupport to nanometric scale was responsible of an increase of surface to volume ratio and a decrease of diffusion distances, thus the interaction kinetic between the capture molecule and the target are improved compared to macro and micro sized support. After the target capture, the nanoparticles and therefore the target are separated from the matrix and preconcentrated in a magnetic chamber before the detection step. Indeed, this magnetic chamber, made of ferromagnetic beads, allow the trapping of the magnetic nanoparticles. Thus, the developed microsystem was able to determine IgE concentration with a 25 fold reduction of assay time as well as a 50 times reduced sample consumption compared to conventional microtiter plate. This last point is crucial since the milk allergy is mainly observed in children. The quantification of IgE in real sera conditions and the results comparison to gold standard validated the developed microsystem as a tool for allergy diagnosis.
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Submitted on : Thursday, July 21, 2011 - 3:21:58 PM
Last modification on : Saturday, September 26, 2020 - 11:44:07 PM
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  • HAL Id : pastel-00610305, version 1

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Bruno Teste. Développement d'un microsystème bioanalytique intégrant des nanoparticules magnétiques dédié au diagnostic de l'allergie. Alimentation et Nutrition. Université Pierre et Marie Curie - Paris VI, 2011. Français. ⟨pastel-00610305⟩

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