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Métabolisme lipidique et cycle du glyoxylate chez la levure Yarrowia lipolytica

Abstract : The yeast Yarrowia lipolytica is an oleaginous yeast which is able to grow on hydrophobic substrates and C2 compounds as a sole carbo source. The first part of this study deals with the localization of the two proteins Lro1p and Dga1p involved in the final step of triglycerides synthesis. These proteins were shown to be localized in the cytoplasmic membrane and at the surface of the lipid bodies for Lro1p and at the lipid bodies surface for Dga1p. The second part of this study helps to decipher about the function of the glyoxylate cycle in yeast Y. lipolytica. The first objective was to understand the function of the malate dehydrogenase gene in the yeast Y. lipolytica. Unlike in the yeast S. cerevisiae which has three genes encoding a malate dehydrogenase (cytoplasmic, mitochondrial and peroxysomale), Y. lipolytica has only two genes. The first gene YALI0D16753g encodes the mitochondrial malate dehydrogenase and the second gene YALI0E14190g is subject to alternatives splicing. Indeed, depends on the splicing of the intron at the 3' splicing site, the gene YALI0E14190g codes for a cytoplasmic malate dehydrogenase (C-terminal sequence PAN) or a malate dehydrogenase targeted into peroxisome (C-terminal sequence AKI). In the third part, we analyzed the other genes of the glyoxylate cycle. The disruption of the gene ICL1 gene (YALI0C16885g) results in the inability of the mutant to growth on oleic acid and C2 compounds (ethanol, acetate). However, the deletion of the others genes of the glyoxylate cycle namely YALI0E15708g (equivalent of MLS1) and YALI0E02684g (equivalent of CIT2) had no impact on growth on media requiring the involvement of the glyoxylate cycle.
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Submitted on : Thursday, September 22, 2011 - 5:50:55 PM
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  • HAL Id : pastel-00625879, version 1
  • PRODINRA : 246089

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Affoue Philomene Kabran-Gnankon. Métabolisme lipidique et cycle du glyoxylate chez la levure Yarrowia lipolytica. Biotechnologies. AgroParisTech, 2010. Français. ⟨NNT : 2010AGPT0060⟩. ⟨pastel-00625879⟩

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