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Quantum Dots pour le Ciblage en Cellules Vivantes et la Microscopie HiLo Bi-couleur

Abstract : Quantum Dots (QDs) are semiconductor nanocrystals with unique optical properties. Their use as probes in biology requires their water solubilization through an adapted surface chemistry which will determine the QD final size, its optical properties and its interaction with the biological environment. We propose a new ligand for QD water solubilization, the dihydrolipoic acid - sulfobetaine (DHLA-SB), which combines simultaneously small size, good stability in a large pH range, in saturated salt solutions and during time and with very low non specific adsorption to cellular membranes. Afterwards we analyzed the intracellular behavior of DHLA-SB QDs during time and we compared it to two other QD classes. This study clearly demonstrated the surface chemistry influence on the intracellular fate of the nanoparticules and also revealed an increased stability of DHLA-SB QDs. We then functionalized the QDs with streptavidin (SA) or biotin to specifically label live and fixed cells. We demonstrated how DHLA-SB QDs-SA are able to efficiently stain a membrane receptor as well as an intracellular biotinylated protein into living cells, with a higher specificity than commercial QDs-SA (Invitrogen). Lastly we proposed to use DHLA-SB QDs as probes for a new structured illumination imaging technique, two-color Hilo microscopy, which allows to obtain an optical section (similar to a confocal image) of a thick biological sample with only one image acquisition.
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https://pastel.archives-ouvertes.fr/pastel-00631485
Contributor : Daniel Swift <>
Submitted on : Wednesday, October 12, 2011 - 2:53:09 PM
Last modification on : Wednesday, October 14, 2020 - 3:43:17 AM
Long-term archiving on: : Monday, December 5, 2016 - 12:16:16 AM

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  • HAL Id : pastel-00631485, version 1

Citation

Eleonora Muro. Quantum Dots pour le Ciblage en Cellules Vivantes et la Microscopie HiLo Bi-couleur. Science des matériaux [cond-mat.mtrl-sci]. Université Pierre et Marie Curie - Paris VI, 2011. Français. ⟨pastel-00631485⟩

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