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Measurement of cicular dichroism dynamics in a temperature jump experiment for the study of protein folding

Abstract : The question how proteins fold into their specific three-dimentional structures provides an exciting challenge for biophysicists nowadays. The use of a fast temperature-jump is a very powerful technique for the study of the denaturation process of proteins. However, probing the secondary structure is a difficult challenge and rarely yields quantitative values. The main purpose of this PhD project is to develop a technical implementation of far-UV circular dichroism in a nanosecond T-jump experiment. Our CD/T-jump experiment allows us to follow quantitatively the change in the helical fraction of a poly(glutamic acid) peptide during its thermal denaturation with 12 ns time resolution.
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https://pastel.archives-ouvertes.fr/pastel-00656201
Contributor : Mai Khuc <>
Submitted on : Tuesday, January 3, 2012 - 5:13:33 PM
Last modification on : Wednesday, January 23, 2019 - 10:29:00 AM
Long-term archiving on: : Wednesday, April 4, 2012 - 2:55:34 AM

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  • HAL Id : pastel-00656201, version 1

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Mai-Thu Khuc. Measurement of cicular dichroism dynamics in a temperature jump experiment for the study of protein folding. Biological Physics [physics.bio-ph]. Ecole Polytechnique X, 2011. English. ⟨pastel-00656201⟩

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