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Réseaux de multicapteurs électrochimiques pour la détection du monoxyde d'azote et de l'anion peroxynitrite en solution

Abstract : Nitric oxide (NO*) and peroxynitrite (ONOO−) are involved in several pathologies, including cancer, Parkinson and Alzheimer diseases, and traumatic brain injury. In this work, we describe the development of electrochemical sensors for the simultaneous detection of these two biologically relevant analytes. To meet this goal, we developed a device integrating several arrays of ultramicroelectrodes (UMEs), using photolithographic techniques. In comparison with individual UMEs, these arrays increase the sensitivity of the measurement, as shown by their electrochemical characterization. Several types of membranes, electropolymerized on top of the electrodes, were tested for their ability to enhance the selectivity of NO* measurement against biological interferents. Those studies allowed us to identify a combination of polyeugenol and polyphenol membranes that improves notably the sensor's selectivity. We also developed a strategy to electrochemically detect ONOO−, based on the reduction of its conjugated acid on bare gold electrodes. Based on those approaches, the simultaneous electrochemical detection of NO* and ONOO− from synthetic solutions was performed. Finally, we describe the detection of NO* produced by the RAW 264.7 macrophages cell line.
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Submitted on : Friday, January 20, 2012 - 3:17:59 PM
Last modification on : Tuesday, September 27, 2022 - 4:16:27 AM
Long-term archiving on: : Wednesday, December 14, 2016 - 12:04:06 AM

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  • HAL Id : pastel-00661741, version 1

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Damien Quinton. Réseaux de multicapteurs électrochimiques pour la détection du monoxyde d'azote et de l'anion peroxynitrite en solution. Chimie thérapeutique. Université Pierre et Marie Curie - Paris VI, 2011. Français. ⟨pastel-00661741⟩

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