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Couches moléculaires sur silicium pour la détection sélective de protéines

Abstract : Biochips are effective analytical tools for medical or defence applications. Protein chips are particularly interesting as proteins are the direct manifestation of cellular activity. These chips are generally constituted of a substrate on which probes, that possess a specific affinity with the targeted proteins, are spotted. However, the current selectivity and sensitivity of these chips is often not sufficient to reliably detect lethal concentrations of some toxins, mainly due to the non specific adsorption of the targets onto the surface, as well as the fragility of the immobilization of the probes. Here we discuss a new way to increase this sensitivity, by using a controlled chemistry on the surface of silicon which, through the grafting of monolayers of alkyl-poly(ethylene glycol) molecules, limits the undesired protein adsorption, as well as enables the covalent immobilization of aptamer probes.
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https://pastel.archives-ouvertes.fr/pastel-00675150
Contributor : Emmanuel Perez <>
Submitted on : Wednesday, February 29, 2012 - 11:20:54 AM
Last modification on : Wednesday, March 27, 2019 - 4:18:02 PM
Long-term archiving on: : Friday, March 31, 2017 - 1:54:24 AM

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  • HAL Id : pastel-00675150, version 1

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Emmanuel Perez. Couches moléculaires sur silicium pour la détection sélective de protéines. Science des matériaux [cond-mat.mtrl-sci]. Ecole Polytechnique X, 2011. Français. ⟨pastel-00675150⟩

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