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Imagerie tridimensionnelle multiphotonique des tissus biologiques à l'aide d'impulsions façonnées

Abstract : This thesis presents the application of ultrashort pulses in the context of multiphoton microscopy and quantum optics. We will first introduce some pulse shaping techniques and we will detail in particular the operation and the characterization of our pulse shaping apparatus. We will study an optically addressed diffractive 2D phase mask, allowing both spectral and phase shaping. Then, we will see how pulse shaping can be an effective tool providing tunable selective fluorophore excitation in two-photon microscopy. Thus, we will present an experiment of coherent control of the 2-photon absorption in a developing drosophila embryo. Afterwards, we will describe a prism-based pulse shaping scheme in conjunction with a time-multiplexing approach of the pulses that allowed the simultaneous acquisition of multiple fluorophores. Ultimately, we will present the application of ultrashort pulses to measure time and dispersion at the standard quantum limit. In particular we will detail theoretically and experimentally a new pulse shaping scheme based on birefringent materials in order to produce the temporal derivative of the electric field or the temporal derivative of its envelope.
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Submitted on : Monday, May 7, 2012 - 2:26:02 PM
Last modification on : Wednesday, January 23, 2019 - 10:29:00 AM


  • HAL Id : pastel-00695044, version 1



Guillaume Labroille. Imagerie tridimensionnelle multiphotonique des tissus biologiques à l'aide d'impulsions façonnées. Optique [physics.optics]. Ecole Polytechnique X, 2011. Français. ⟨pastel-00695044⟩



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