Biopuces sans marquage : structuration pour la haute sensibilité pour l'imagerie dans l'ultraviolet

Kristelle Robin-Bougot 1
1 Laboratoire Charles Fabry de l'Institut d'Optique / Naphel
LCFIO - Laboratoire Charles Fabry de l'Institut d'Optique
Abstract : Biosensors enables to detect biological interactions, between probes localized at the chip surface, and targets of a solution. Biological applications of biosensing are wide. Here, we present a label-free optical transduction, enabling 2D imaging, and consequently parallel detection of several reactions. It is based on the absorption of biological molecules (at 260 nm for DNA and 280 nm for proteins). In this framework, recently developed AlGaN components can be used. Their emission/responsivity spectrum enables to select the spectral band of interest. Sensitivity is a major requirement of biosensing devices. Configurations leading to enhancement of the interaction between light and biological molecules are of interest. The first multilayer structures enable to locate the biological molecules at the antinode of the electric field. For a better sensitivity, resonant grating structures are then studied. They enable a much better confinement of the electric field close from the biological layer. The protein used in this study is the methionyl-tRNA synthetase. Its absorption is representative of protein absorption, and it can then serve as a model for biological macromolecules detection. The successive steps of chip modelling, fabrication, characterization, biological preparation and then imaging of the chips are described. Imaging of resonant grating is not largely studied, but it results in good sensitivity. In order to increase signal to noise ratio, a pre-dispersed illumination is proposed. It enables to take benefit of all the useful photons of the source by illuminating the chip in () resonant condition for each wavelength.
Document type :
Theses
Complete list of metadatas

Cited literature [60 references]  Display  Hide  Download

https://pastel.archives-ouvertes.fr/pastel-00718042
Contributor : Marion Avignon <>
Submitted on : Monday, July 16, 2012 - 8:34:01 AM
Last modification on : Thursday, January 11, 2018 - 6:26:11 AM
Long-term archiving on : Wednesday, October 17, 2012 - 2:25:10 AM

Identifiers

  • HAL Id : pastel-00718042, version 1

Citation

Kristelle Robin-Bougot. Biopuces sans marquage : structuration pour la haute sensibilité pour l'imagerie dans l'ultraviolet. Optique [physics.optics]. Université Paris Sud - Paris XI, 2009. Français. ⟨pastel-00718042⟩

Share

Metrics

Record views

307

Files downloads

570