Développement de nouvelles méthodologies de préconcentration électrocinétique in-situ en électrophorèse capillaire pour l'analyse de traces

Abstract : Capillary electrophoresis is a powerful separation technique, but it suffers from a lack of sensitivity when optical detectors are used. In order to resolve this problem, in-situ electrokinetic preconcentration techniques are available. During these researches, some of these techniques had been investigated. First of all, it was shown the coating of silica capillary walls by linear polyacrylamide reduced efficiently the electroosmotic flow even in complex acidic media which improve the electrokinetic preconcentration yields. Secondly, the hyphenation of field amplification with electrokinetic injection and "sweeping" was examined. The mechanism of this technique was deeply studied thanks to chemometric approaches and simulation. The results allowed to clarify the influence of experimental parameters and to simplify the optimization procedure. Following these results, an analytical procedure for the analyses of herbicides in tap water was developed and detection near to the specified level of European Union was obtained without any extraction procedure. Thirdly, it was shown the utilization of ionic liquids in the so-called technique "Micelle to Solvent Stacking" provided sensibility enhancements of 10 by comparison with classical surfactants (application to herbicides and anti-inflammatory drugs). Finally, thanks to the use of a specific aptamer, a proof of concept was developed for the selective preconcentration of Ochratoxine A in wine. This last work open new perspectives for the utilization of these biological tools in capillary electrophoresis.
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Submitted on : Tuesday, October 23, 2012 - 3:35:59 PM
Last modification on : Thursday, July 4, 2019 - 11:00:06 AM
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  • HAL Id : pastel-00744659, version 1

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Philippe Anrès. Développement de nouvelles méthodologies de préconcentration électrocinétique in-situ en électrophorèse capillaire pour l'analyse de traces. Chimie analytique. Université Pierre et Marie Curie - Paris VI, 2012. Français. ⟨pastel-00744659⟩

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