Effet des nanoparticules de dioxyde de titane sur les métalloprotéases, influence des paramètres physicochimiques

Abstract : Exposure to titanium dioxide (TiO2) nanoparticles (NP) can lead to matrix remodeling, a phenomenon in which Matrix metalloprotease-1 (MMP-1) notably could play an important role. To validate this hypothesis, we 1/ studied MMP-1 expression in human pulmonary fibroblasts after exposure to TiO2 particles of different sizes, shapes and crystal phases and one Carbon Black (CB) NP, and 2/ measured the effect of TiO2 CB NP on an elastase-induced emphysema model in rats.On human lung fibroblasts, some TiO2 NP induced MMP-1 through an interleukin-1β (IL-1β) dependent mechanism, and increased MMP-1 activity. TiO2 micrometric particles induced MMP-1 without IL-1β implication, and CB NP did not induce MMP-1. Rat exposure to TiO2 NP had no effect but exposure to NC NP induced inflammation and MMP-12 expression. TiO2 NP did not modulate elastase effects. NC NP aggravated elastase-induced inflammation.In total, our work shows a role for TiO2 NP in matrix remodeling, via MMP-1 induction and activation in vitro. In vivo, CB NP can aggravate some elastase-induced effects. Particles composition and size seem to be important determinants of their effect.
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Lucie Armand. Effet des nanoparticules de dioxyde de titane sur les métalloprotéases, influence des paramètres physicochimiques. Toxicologie. AgroParisTech, 2011. Français. ⟨NNT : 2011AGPT0063⟩. ⟨pastel-00771963⟩

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