H3S10P, Phosphorylation at Ser10 in Mouse Preimplantation Embryos

Abstract : Pericentromeric heterochromatin appears to be involved with gene regulation and therefore with the developmental potential of embryos. We hypothesized that an epigenetic modification, H3S10P, could be a new marker to follow pericentromeric heterochromatin in preimplantation mouse embryos. Using immunofluorescence, immunoFISH and high resolution microscopy, we observed that H3S10P shows a different distribution pattern in mouse embryos than in somatic cells. It is detected early in interphase around the Nucleolar-Precursor Bodies from 1- to 4-cell, in co-localization with the DNA probes for pericentromeric heterochromatin, and is seen in the chromosome arms throughout mitosis. In fact, H3S10P shows a similar kinetic as seen in somatic cells only after the 4-cell stage: being solely observed in the chromocenters during late interphase and on the mitotic chromosomes until telophase. This distribution seems related to the absence of Aurora B kinase in the earlier stages. We have also compared H3S10P to other related pericentromeric heterochromatin markers such as H3K9me3, HP1β and the double modification, H3K9me3S10P, and concluded that H3S10P is a better marker for pericentromeric heterochromatin of both parental origins. Finally, as cloned embryos often show abnormal pericentromeric heterochromatin remodelling and impaired development after Somatic Cell Nuclear Transfer (SCNT), H3S10P was used to track down heterochromatin reprogramming after SCNT. Our results show that H3S10P underlines only the portion of heterochromatin which is remodelled when compared with the other related markers and that the unremodelled portion maintains the epigenetic signature of the donor cell.
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Karlla Ribeiro de Sousa. H3S10P, Phosphorylation at Ser10 in Mouse Preimplantation Embryos. Agricultural sciences. AgroParisTech, 2011. English. ⟨NNT : 2011AGPT0061⟩. ⟨pastel-00813347⟩

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