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Clonage et expression des prochymosines bovines A, B et B S79N chez Escherichia coli et Pichia pastoris. Etude de la mutation S79N

Abstract : Chymosin, an enzyme used in cheese manufactures as a milk-clotting agent, is traditionaly prepared from the fourth stomach of unweaved calves. This crude preparation called rennet has been substituted by microbial and plant coagulants for years since chymosin production was unable to satisfy the world demand especially due to the increase of cheese production. However, chymosin still remains the better milk-clotting enzyme. Thus, genetic engineering techniques are used to produce recombinant bovine chymosin. Using these techniques, we propose to establish the conditions for production of recombinant bovine chymosin, allowing technological innovation and contributing to the development of food industry of Brazil and countries of the Economic Market of “Cone Sul” (MercoSul).
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Submitted on : Tuesday, November 30, 2004 - 4:39:40 PM
Last modification on : Saturday, April 20, 2019 - 1:23:46 AM
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  • HAL Id : tel-00005681, version 1

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Alexandra Abreu da Silva. Clonage et expression des prochymosines bovines A, B et B S79N chez Escherichia coli et Pichia pastoris. Etude de la mutation S79N. Biochimie [q-bio.BM]. INAPG (AgroParisTech), 2004. Français. ⟨NNT : 2004INAP0001⟩. ⟨tel-00005681⟩

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