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Etudes de systèmes microfluidiques : agrégation de particules, électrocinétique linéaire, analyse de protéines

Abstract : This manuscript is divided in two introductory chapters and three main parts : (I) The aggregation of paramagnetic particles in the presence of an hydrodynamic shear is studied experimentally. Chains formed growth linearly with time following a theoretical model based on Smoluchovsky equation. (II) Theoretical works extend electrokinetics relations in order to describe various situations proposed by microfluidics devices. Symetries between electroosmosis and streaming current is prooved. Thin Debye Layer approximation is extended to streaming current and applied to describe electrical and velocity field in typical geometries. (III) A “lab on a chip” is presented allowing identification of protein sample. The first level performs electrophoretic separation. The second digests selected proteins using an enzyme. Peptides obtained are then analyzed by mass spectrometry.
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https://pastel.archives-ouvertes.fr/tel-00007776
Contributor : Edouard Brunet <>
Submitted on : Monday, December 27, 2004 - 2:43:49 PM
Last modification on : Friday, July 17, 2020 - 8:51:16 AM
Long-term archiving on: : Monday, September 20, 2010 - 12:15:13 PM

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  • HAL Id : tel-00007776, version 2

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Edouard Brunet. Etudes de systèmes microfluidiques : agrégation de particules, électrocinétique linéaire, analyse de protéines. Biophysique [physics.bio-ph]. Université Pierre et Marie Curie - Paris VI, 2004. Français. ⟨tel-00007776v2⟩

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