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ADN double brin (315 paire de bases) biotinylé aux deux extrémités est synthétisé à l'aide du kit PCR (Expand High Fidelity PCR System) fourni par Roche Applied Science en utilisant des amorces biotinylées (Eurogentec) et un ?-ADN comme ADN de départ, ADN final est purifié et sa concentration est mesurée par fluorescence ,
L'échantillon est centrifugé à 400g et les cellules sont redispersées dans 500µL de tampon PBS contenant un tensio-actif pluronic (F-127) à 0 ,