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Theses

Transfert de gène in vivo:
étude, régulation et application de l'électrotransfert

Abstract : We have been dealing with different projects using a physical method of in vivo gene transfer,
called electrotransfer, widely used in mouse muscle and skin with high gene expression.
By in vivo optical imaging, we have been working on optimization of electrotransfer. We have
developed an artificial regulation system to in vivo control an exogenous gene expression based on
the antisense technology using muscular electrotransfer in mouse. We have worked on molecular
optimization of DNA electrotransfer for genetic immunization for botulinum antigen screening.
This kind of DNA immunization has been used to induce antibodies production in mouse and
rabbit. We have been studying the methylation status and the reasons for persistence of plasmid
DNA in muscle cells after electrotransfer since intramuscularly injected and electrotransferred
plasmid DNA leads to a persisting expression for more than a year.
Complete list of metadatas

https://pastel.archives-ouvertes.fr/tel-00011410
Contributor : Capucine Trollet <>
Submitted on : Wednesday, January 18, 2006 - 12:03:36 PM
Last modification on : Tuesday, September 22, 2020 - 3:58:38 AM
Long-term archiving on: : Tuesday, June 15, 2010 - 4:28:11 PM

Identifiers

  • HAL Id : tel-00011410, version 1

Citation

Capucine Trollet. Transfert de gène in vivo:
étude, régulation et application de l'électrotransfert. Biochimie [q-bio.BM]. Chimie ParisTech, 2005. Français. ⟨tel-00011410⟩

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