Skip to Main content Skip to Navigation
Theses

la bioluminescence de l'aequorine en réponse au calcium In vitro et dans le Cortex cerebral

Abstract : During my PhD, I investigated in vitro the calcium-dependent bioluminescence of thephotoprotein aequorin and then used its bioluminescence to image neuronal activities in theneocortical network. This genetically encoded calcium sensor can be expressed in specific cell types and its bioluminescence is not toxic and exhibit a high signal/noise ratio.I first search for mutations modifying aequorin bioluminescence, using a random
mutagenesis and in vitro evolution approach. I isolated mutants showing modified stability,calcium sensitivity and/or luminescence kinetics. The study of these mutants disclosedrelationships between bioluminescence kinetics and calcium sensitivity of aequorin. These results help to understand how calcium binding leads to photon emission by aequorin.Next, I used the bioluminescence of a GFP-aequorin chimera expressed in
neocortical slices with the recombinant Sindbis virus to image the activities of neuronalensembles. Using this approach, I studied the cholinergic modulation of neocortical responses to electrical stimulation. I showed that muscarinic agonists increase spatial extent and the duration of neocortical network responses to electrical stimulations
Document type :
Theses
Complete list of metadatas

https://pastel.archives-ouvertes.fr/tel-00116205
Contributor : Francoise Tchang <>
Submitted on : Friday, November 24, 2006 - 5:16:49 PM
Last modification on : Wednesday, October 14, 2020 - 3:43:08 AM
Long-term archiving on: : Tuesday, April 6, 2010 - 7:59:57 PM

Identifiers

  • HAL Id : tel-00116205, version 1

Citation

Ludovic Tricoire. la bioluminescence de l'aequorine en réponse au calcium In vitro et dans le Cortex cerebral. Neurosciences [q-bio.NC]. Université Pierre et Marie Curie - Paris VI, 2006. Français. ⟨tel-00116205⟩

Share

Metrics

Record views

1384

Files downloads

10944