Développement et organisation de nanostructures :
applications à l'exaltation des processus optiques pour la Biologie

Abstract : Molecule fluorescence is subject to the influence of its electromagnetic environment. Excitation and emission processes can be modified by the presence of a metallic structure, due to interference phenomena and coupling to surface plasmons. We elaborated and characterized metal films ranging in morphology from nanoparticles to percolated, continuous, plane and rough films. Their influence on fluorophore optical behavior was investigated by experiment and theoretical model, as a function of fluorophore-to-metal distance and molecular orientation. The detected signal is found to be amplified by one to two orders of magnitude. We moreover report fluorophore photostabilization and the modification of intermolecular energy tranfer processes. We demonstrate the interest in this technology for sensitivity improvement of DNA chip and for application in cell and tissue imaging.
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Submitted on : Friday, July 20, 2007 - 4:41:17 PM
Last modification on : Sunday, March 31, 2019 - 1:19:22 AM
Document(s) archivé(s) le : Thursday, April 8, 2010 - 11:43:29 PM

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  • HAL Id : tel-00164549, version 1

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Eric Le Moal. Développement et organisation de nanostructures :
applications à l'exaltation des processus optiques pour la Biologie. Biophysique [physics.bio-ph]. Université Pierre et Marie Curie - Paris VI, 2007. Français. ⟨tel-00164549⟩

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