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Theses

Nanoparticules à luminescence persistante pour l'imagerie optique in vivo

Abstract : The goal of this work was to develop and use a new kind of optical probes that have the properties of persistent luminescence. Commonly referred as phosphorescence, persistent luminescence is the phenomenon encountered in materials which make them glow in the dark after the end of an excitation with UV or visible light.

Due to growing demands of imaging tools for biomedical research, existing imaging systems have been rapidly improved and new imaging techniques have been developed during the past decades. Nowadays, imaging techniques such as MRI, scanner, ultrasound, scintigraphy and other major imaging systems are available to scientists. Each technique has advantages and disadvantages, making them complementary.

Optical imaging is a rapid expanding field with direct applications in pharmacology and in the development of tools for diagnostic and research in molecular and cellular biology. Despite the increasing use of fluorescence for in vivo imaging, this technique presents several limitations, especially due to tissues autofluorescence under external illumination and weak tissue penetration of low wavelength excitation light. These drawbacks can limit the ability to detect fluorescent probes from background signal in deep tissues imaging.

We present here an alternative optical imaging system using near-infrared persistent luminescence (commonly called phosphorescent) nanoparticles suitable for small animal imaging. The main advantage of this technique resides in the absence of autofluorescence, as the nanoparticles continue to emit light in the animal without the need for any kind of excitation.

Using these probes in small animal imaging, we demonstrate that nanoparticles can be excited prior to injection to follow their in vivo distribution for more than one hour without any external illumination source. Chemical modification of nanoparticles surface led to distinct biodistribution pattern. Tumor mass was also identified on a mouse model.
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https://pastel.archives-ouvertes.fr/tel-00294982
Contributor : Quentin Le Masne de Chermont <>
Submitted on : Thursday, July 10, 2008 - 10:41:32 PM
Last modification on : Wednesday, October 14, 2020 - 3:58:06 AM
Long-term archiving on: : Monday, October 1, 2012 - 11:00:46 AM

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  • HAL Id : tel-00294982, version 1

Citation

Quentin Le Masne de Chermont. Nanoparticules à luminescence persistante pour l'imagerie optique in vivo. Matériaux. Chimie ParisTech, 2007. Français. ⟨tel-00294982⟩

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