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Caractéristiques Optiques et Dynamiques d'Interactions Biomoléculaires de Surface - Application aux biopuces à ADN

Jérôme Hottin 1
1 Laboratoire Charles Fabry de l'Institut d'Optique / Macsybio - bio
LCFIO - Laboratoire Charles Fabry de l'Institut d'Optique
Abstract : As medical science advances towards the generalisation of predictive methods, biosensors are taking on the lead role. Based on a variety of detection and transduction methods, different biosensors are being developed in order to observe biomolecular interactions. The scope of application is vast: detection of pollutants or genetically modified organisms, improvement of drug specificity (anti-cancer treatment) or reliable genetic diagnosis. My research is focused on the detection of the Cystic Fibrosis genetic disease. The biosensor that we used relies on an optical system using SPR imaging coupled to a DNA biochip. The probe molecules are single strands of synthesized DNA whose sequence was calculated in order to be able to detect, in the patient's target DNA single strands, the genetic modifications of the CFTR gene responsible for the disease. These biochips consist of a metallic layer functionalized by surface chemistry based on the thiol/gold bond and ending in a layer of Streptavidin. The probes are biotinilyzed and a micro-arrayer spots them out in the form of a matrix on the surface. Through the implementation of a temperature control module and various studies on the TM of DNA-DNA interactions, along with a data correction method, we succeeded in establishing the genetic diagnosis of a SNP without ambiguity in around ten minutes. The last studies of this document pave the way to the experimental cartography of the secondary structures of target single strands.
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https://pastel.archives-ouvertes.fr/tel-00430804
Contributor : Jérôme Hottin <>
Submitted on : Monday, November 9, 2009 - 11:09:17 PM
Last modification on : Wednesday, October 14, 2020 - 3:56:46 AM
Long-term archiving on: : Thursday, June 17, 2010 - 6:16:11 PM

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  • HAL Id : tel-00430804, version 1

Citation

Jérôme Hottin. Caractéristiques Optiques et Dynamiques d'Interactions Biomoléculaires de Surface - Application aux biopuces à ADN. Biophysique [physics.bio-ph]. Université Paris Sud - Paris XI, 2009. Français. ⟨tel-00430804⟩

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