Milli-fluidique digitale pour la microbiologie

Abstract : In France, we all know one of the Ministry of Health slogans: "antibiotics are not automatic." The main objective of this campaign was to inform people so they have a better use of antibiotics in order to preserve their effectiveness. Indeed, over-consomation of antibiotics has encouraged the emergence of mutant organisms more resistant to treatment. But the mutations are usually long process. Before even mutate, organizations have the ability to resist antibiotic underdosing. This resistance is measured in the application in pharmacology. Measures used to determine the methods revealed in antibiotic dosage, but with a very low resolution on concentration. Technological advances in the field of miniaturization since the 1980s have helped to generate structures confined to the micron scale. These methods technically meet necessitated in the field of microbiology. Indeed, the micro-fluidic chips enable transport and handling of very small amounts of samples. These chips integrate series of successive operations for biological analysis. Micro-fabrication are also used to produce a controlled manner and periodic drops very monodisperse emulsion. In these drops can retain biological compounds of interest. A major focus of microfluidics is the use of emulsion droplets as bio-reactor. This means that applications of digital microfluidic technologies are diversifying in the field of microbiology. In this thesis, we developed a scaling tool than microfluidics. This tool is part of technical processes named milli-fluidics. Routes developed consist of capillaries of sub-millimeter section connected to each other by means of machined parts for this purpose. Although this area is a ladder on top of microfluidics, it is governed by the same laws as microfluidics. We focused on the transposition in milli-fluidic micro-fluidic functions, including on the co-encapsulation of organizations and antibiotics gout. This in order to measure the bacterial antibiotic activity due to different stress intensities, by the development of an automated instrument.
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Fabien Bertholle, Jérôme Bibette. Milli-fluidique digitale pour la microbiologie. Chimie analytique. Université Pierre et Marie Curie UPMC Paris VI, 2010. Français. ⟨tel-01116366⟩

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