Métabolisme du carbone et virulence chez Neisseria meningitidis

Abstract : Neisseria meningitidis has an incomplete PTS composed of general proteins EI and HPr and two EIIAs (EIIANtr and EIIAMan). This system does not allow the transport of sugars in this bacterium. However, we confirmed that the phosphorylation cascade (EI HPr EIIANtr) is functional and HPr is also phosphorylated at its Ser-46 by an HprK/P.In order to study the effect of HPr on meningococcal virulence, we constructed a ΔptsH mutant in N. meningitidis 2C4-3. Compared to the wild-type strain, the ΔptsH mutant showed poor survival in mice, low production of capsule, better adherence to epithelial cells and high levels of apoptotic cells. HPr appears to be involved in the virulence of N. meningitidis by interacting with CrgA protein. The HPr/CrgA interaction is stronger when HPr is phosphorylated at its Ser-46 by HprK/P.N. meningitidis uses glucose and maltose as the only sugars. We identified permeases for glucose (GlcP) and maltose (MalY), which catalyze the uptake of these sugars.A putative gluconate permease (GntP) has also been identified in N. meningitidis 2C4-3. Under the conditions tested, this permease did not catalyze the transport of gluconate. Compared to the wild-type strain, deletion of gntP in N. meningitidis 2C4-3 induced faster growth on glucose and a better survival of the mutant in mice. To underst and the function of the GntP permease in N. meningitidis further studies will have to be carried out.
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Meriem Derkaoui. Métabolisme du carbone et virulence chez Neisseria meningitidis. Sciences agricoles. AgroParisTech, 2015. Français. ⟨NNT : 2015AGPT0047⟩. ⟨tel-01418379⟩

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