Développement d'un instrument plasmonique bimodal couplant SPRI et SERS pour la détection et l'identification de molécules biologiques

Abstract : Surface Plasmon Resonance Imaging (SPRI) is a powerful technique to study molecular interactions providing a real time, label free and high throughput analysis. The transduction of an interaction between complementary molecules into an optical signal is based on the perturbation of a plasmonic evanescent wave supported by a thin metallic film.However, despite its direct and label free assets, the specificity of SPR measurements is only guaranteed by the probe molecules grafted on the metallic surface and therefore by the quality of the surface chemistry. This limitation becomes an issue when addressing major health concerns relying on the detection of trace molecules. In particular, new systems are required to help early diagnosis and the control of food contaminants.In view of improving measurement’s specificity, this work reports the development of a bimodal instrument coupling SPRI, allowing the quantification of captured molecules, with Surface Enhanced Raman Spectroscopy (SERS), adding the precise identification of the molecules by measuring their spectroscopic fingerprint. This PhD is part of an ANR project bringing together academic and industrial partners.This manuscript focuses on the development of the optical instrument combining the two detection systems in a unique prototype. SPRI measurements are performed in the Kretschmann configuration while SERS analysis is implemented from the top, in solution, through a glass window. Nanostructured substrates have been designed and realized to allow the simultaneous experiment.The optical system is described, characterized and validated on the model case of a DNA hybridization. These first results prove the capabilities of the bimodal instrument in the perspective of more complex biological applications.
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Aurore Olivéro. Développement d'un instrument plasmonique bimodal couplant SPRI et SERS pour la détection et l'identification de molécules biologiques. Optique [physics.optics]. Université Paris-Saclay, 2016. Français. ⟨NNT : 2016SACLO017⟩. ⟨tel-01469228⟩

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