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Un mélange d'hybridation (« annealing ») est constitué de volume final 2X µL, X étant le nombre d'expériences à réaliser. Pour 15 expériences par exemple, il faut prévoir un volume de 30µL de composition suivante : 1picomole d'ARNm purifié, 3pmoles d'oligonucléotide amorce, ) ; on complète à 30µL par de l'eau MQ. La solution est chauffée 3min à 60°C ,
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ARNt cible. L'oligo est marqué en 3'-biotin et 5'-atto488. 2 à 3 adénosine sont rajoutées à l'extrémité 3' pour faciliter la liaison aux billes ,
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