Droplet-based Microfluidic Platform for Quantitative Microbiology

Abstract : Development of a microfluidic chip for quantitative microbiology. The chip allow for parallel culture of thousands bacterial colonies in micro-droplets stored in static array. The 2D-array enable not only the visualisation of each colonies in timelapse experiment but also the extraction of any of them out of the chip at any time for further analysis (PCR, re-culture,...). The platform is adaptable to a concentration gradient producer, for which we present the physical understanding of working mechanism, that can apply different chemical environments to each colony. We developed in parallel a software that perform the analysis of the data generated by the platform to adress bacteria growth studies as well as the impact of antibiotics on bacteria proliferation.
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  • HAL Id : tel-01514194, version 1

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Cyprien Guermonprez. Droplet-based Microfluidic Platform for Quantitative Microbiology. Micro and nanotechnologies/Microelectronics. Université Paris-Saclay, 2016. English. ⟨NNT : 2016SACLX106⟩. ⟨tel-01514194⟩

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