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Détection d'acides nucléiques avec des réseaux de transistors à effet de champs à base de graphène

Abstract : This work focuses on using graphene field effect transistors (GFETs) arrays for electronic DNA hybridization detection.In the first part, we use fluorescence based detection to study some factors affecting DNA hybridization such as temperature, buffer ionic strength, probes and targets concentration and hybridization duration. Optimum conditions for DNA hybridization have been found by using this technic. The second part of this work is devoted to the chip fabrication. Original methods for Cu-CVD grown graphene transfer and photolithography process are developed on Si/SiO2 substrate. Device’s performance is highly improved after annealing in vacuum at 280°C for 24 hours if needed. Passivation layers are used for time stability and sensitivity. In the third section we perform electronic detection. Single stranded DNA (ssDNA) are immobilized on a chip thanks to polylysine (PLL) adsorption. This positively charged polymer adsorption induces a right shift of the Dirac point whereas probes fixation trigger a left shift. Complementary targets (cDNA) hybridization is accomplished by differential measurements and real time in situ measurement.
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  • HAL Id : tel-02631689, version 1

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Kokoura Mensah. Détection d'acides nucléiques avec des réseaux de transistors à effet de champs à base de graphène. Biophysique. Université Paris sciences et lettres, 2019. Français. ⟨NNT : 2019PSLET010⟩. ⟨tel-02631689⟩

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