Skip to Main content Skip to Navigation
Theses

Développement du séquençage ARN ciblé sur cellules uniques en microfluidique de gouttes et applications

Abstract : Single cells technologies were introduced a few years ago and have been dramatically evolving ever since. These technologies have revolutionized biology, making it possible to better understand how heterogeneous cell systems works. For example, they permit to discover and follow cell subtypes, with applications in oncology or neurobiology. We have developed a technology to study the expression profile of genes of interest at the level of a single cell, using droplet-based microfluidics. By limiting the number of genes studied compared to commercial whole-transcriptome technologies, the targeted approach has several potential benefits: gaining deeper sequencing, increasing the number of cells studied, optimizing detection for low levels of expression, while reducing the complexity of data and costs. Targeting is sometimes essential, especially when the RNAs do not carry a generic primer sequence, as in the case of viral RNAs. Two applications are presented: the analysis of inflammation of the immune cells of the brain in the early stages of development, as well as the study of genetic recombination in the virus.
Document type :
Theses
Complete list of metadatas

Cited literature [300 references]  Display  Hide  Download

https://pastel.archives-ouvertes.fr/tel-02923561
Contributor : Abes Star :  Contact
Submitted on : Thursday, August 27, 2020 - 11:27:11 AM
Last modification on : Wednesday, October 14, 2020 - 3:43:04 AM

File

ESPCI_SophieFOULON_2019.pdf
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-02923561, version 1

Citation

Sophie Foulon. Développement du séquençage ARN ciblé sur cellules uniques en microfluidique de gouttes et applications. Chimie théorique et/ou physique. Université Paris sciences et lettres, 2019. Français. ⟨NNT : 2019PSLET037⟩. ⟨tel-02923561⟩

Share

Metrics

Record views

51

Files downloads

57