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Analyse non ciblée des biotoxines marines dans les produits de la pêche

Abstract : Considering the globalisation of marine biotoxin distribution, the emergence of new toxins and the proven risk to human health, it is essential to have tools that are sufficiently versatile and effective to detect the widest possible range of known or emerging toxins in order to guarantee consumers’ safety. This PhD falls within the scope of the monitoring of marine toxins. It aims to contribute to the evolution of the vigilance system by implementing a non-targeted approach based on the use of high-resolution mass spectrometry as an alternative to the use of the controversial mouse bioassay.First, a liquid chromatography high-resolution mass spectrometry method was developed and characterized for the analysis of 32 marine toxins with a wide range of polarities, using a hybrid quadrupole time-of-flight (QTOF) mass spectrometer. Reversed-phase and hydrophilic interactions columns have been used for the separation of targeted lipophilic and hydrophilic toxins. A strategy describing the different steps of a non-targeted approach, from acquisition to data processing, by chemometric tools was then developed using two different softwares (the SCIEX software suite, commercially available, and XCMS, an open source software). The developed workflow was succefully tested as part of a proof, on blindly analysed spiked samples (mussels and oysters) with different toxins. The strategy was then applied to naturally contaminated samples involved in human intoxications and related to the consumption of sea squirts of the Microcosmus genus using two approaches to process the data generated by the non-targeted analysis; the first one consists in screening a list of suspects (suspect screening) and the second one is an analysis without a priori.
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Inès Dom. Analyse non ciblée des biotoxines marines dans les produits de la pêche. Chimie analytique. Institut agronomique, vétérinaire et forestier de France, 2018. Français. ⟨NNT : 2018IAVF0026⟩. ⟨tel-02941624⟩

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