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La réponse au NO au centre de la pathogenèse bactérienne et cible d’antibiotiques innovants

Abstract : NO or nitric oxide is a highly reactive free radical that is ubiquitous in the living world. It is capable of modifying DNA bases, membrane lipids, the activity of enzymes, etc. It has many biological roles in animals, plants, fungi and bacteria. It is produced in particular by cells of the innate immunity to fight against infections.To infect host, certain pathogenic bacteria have developed mechanisms to resist this stress. Bacillus cereus, is a pathogen that is able to resist NO. However, these mechanisms have not been fully elucidated. The Mfd protein is known to repair damage to NO on DNA and to increase resistance to NO stress.On one hand, a transcriptomic approach on Bacillus cereus allows to highlight a genomic region particularly activated in presence of NO. This region contains genes that code for a damage repair protein, a nitrogen metabolism enzyme and enzymes from the biosynthesis of siroheme, a ferrous group.On the other hand, a Mfd inhibitor has been tested for its antibacterial efficiency on Bacillus cereus and on four bacteria responsible for infections in therapeutic impasse due to the appearance of antibiotic resistance.
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Submitted on : Friday, March 19, 2021 - 7:10:08 PM
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Constance Porrini. La réponse au NO au centre de la pathogenèse bactérienne et cible d’antibiotiques innovants. Médecine humaine et pathologie. Université Paris-Saclay, 2020. Français. ⟨NNT : 2020UPASA008⟩. ⟨tel-03175216⟩

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