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Descripteurs physico-chimiques de la biomasse lignocellulosique en hydrolyse enzymatique : vers une caractérisation in-situ

Abstract : One of the paths that are envisioned to reduce the environmental footprint of human’s activities in the field of the transportation is about ethanol production from lignocellulosic biomass. Amongst all the different processes that are projected to convert such substrates made of cellulose, hemicelluloses, and lignin, the bioconversion process into ethanol represents a promising option since it is quite mature, and ethanol is readily usable in the current gas-cars fleet. The pretreatment step that aims at making cellulose more accessible and more digestible to the enzymatic hydrolysis still needs improvements in order to make this process economically viable. To study the relations between operating conditions, physicochemical modifications, and their effects on substrates’ digestibility, a wide range of samples with a high variability of their physicochemical parameters was needed. Samples were produced from a wheat straw that was dilute-acid and steam exploded with a wide range of operating conditions. Multi-technique and multi-scale analyses led to a precise characterisation of the physicochemical effects provoked by the pretreatment. These results also allowed identifying key substrate parameters that are responsible for the substrate enzymatic digestibility. Based on the observations, an empirical model was proposed to express the substrate digestibility as a function of these critical parameters. Finally, in situ monitoring of tissue degradation and enzyme adsorption-desorption on plant tissues during saccharification was carried using UV-autofluorescence microscopy. Thanks tothese experiments, hydrolysis mechanisms on pretreated matrices are now better understood, and depend upon pretreatment operating conditions.
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Submitted on : Friday, April 16, 2021 - 11:53:27 AM
Last modification on : Friday, August 5, 2022 - 2:38:11 PM
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  • HAL Id : tel-03200181, version 1



Joël Passicousset. Descripteurs physico-chimiques de la biomasse lignocellulosique en hydrolyse enzymatique : vers une caractérisation in-situ. Chimie-Physique [physics.chem-ph]. Institut agronomique, vétérinaire et forestier de France, 2019. Français. ⟨NNT : 2019IAVF0004⟩. ⟨tel-03200181⟩



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