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Écologie chimique inverse appliquée aux récepteurs olfactifs de l'insecte ravageur des cultures Spodoptera littoralis : vers des stratégies innovantes en protection des cultures

Abstract : Insects detect, via their odorant receptors (OR), a variety of volatile compounds found in the environment and use them to carry out vital activities such as identifying food sources and sexual partners. The identification of attractive or repellent semiochemicals that interfere with crop pest behaviors is a major issue for their biocontrol. Yet, their application for the control of pest Lepidoptera mainly targets adults, whereas the caterpillars represent the feeding stage.In this context, my work consisted in searching semiochemicals active on the behavior of caterpillars in a model crop pest, the moth Spodoptera littoralis, using a reverse chemical ecology targeting the larval ORs. First, a transcriptomic study allowed us to identify the repertoire of chemosensory genes expressed in the larva. Then, the function of some ORs was determined via their heterologous expression in Drosophila, in order to identify which olfactory cues are detected by larvae. Last, we combined in silico ligand-targeted modeling, functional analyzes of ORs expressed in Drosophila and behavioral assays to identify new ligands for the targeted ORs that are active on the larval behavior.This work validates the use of ORs as targets to accelerate the discovery of new semiochemicals active on behaviors, which, in the long term could be used for the bioncontrol of herbivorous caterpillars. The data obtained also provide fundamental knowledge in the field of neurobiology and the ethology of larvae.
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  • HAL Id : tel-03399743, version 1

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Gabriela Caballero Vidal. Écologie chimique inverse appliquée aux récepteurs olfactifs de l'insecte ravageur des cultures Spodoptera littoralis : vers des stratégies innovantes en protection des cultures. Sciences agricoles. Institut agronomique, vétérinaire et forestier de France, 2020. Français. ⟨NNT : 2020IAVF0011⟩. ⟨tel-03399743⟩

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